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High Resolution X-Ray: A Reliable Approach for Quantifying Osteoporosis in a Rodent Model

机译：高分辨率X射线：在啮齿动物模型中定量骨质疏松症的可靠方法

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Osteoporosis is the most common metabolic disease of bone, resulting in significant worldwide morbidity. Currently, there are insufficient imaging modalities available to evaluate osteoporotic bones in small animal models. Here, we demonstrate the feasibility of using high resolution X-ray imaging as a comparable measure of bone degeneration to dual-energy X-ray absorptiometry (DXA) in an osteoporosis rodent model. At week 0, animals underwent either an ovariectomy (OVX) or sham procedure (SHAM). DXA analysis was performed weekly to confirm and compare the bone degenerative changes induced by OVX. A comparison using high resolution X-ray imaging (Faxitron?) was then performed postmortem due to need of soft tissue removal. Two regions of interest (ROIs) were utilized: the distal third of the femur and the lumbar spine (L4/L5). It was observed that SHAM animals maintained a relatively constant bone mineral density (BMD), in comparison to OVX animals, whereby a significant decrease in BMD was appreciated. Post mortem X-ray scans were performed and converted to 8-bit color and quantified. A high level of agreement with DXA quantifications was observed with X-ray quantifications, and a significant correlation between the radiopacity, visualized by color distributions, and the DXA BMD values between animal groups was evident. Our study demonstrates the applicability of high resolution X-ray imaging both qualitatively and quantitatively as a reliable approach for quantifying osteoporosis in rodent osteoporotic models. With DXA being a highly user dependent modality, our technique is a unique secondary methodology to verify DXA findings and minimize inter-observer variability.
机译：骨质疏松症是最常见的骨骼代谢疾病，导致全世界范围内的高发病率。当前，在小动物模型中没有足够的成像方法来评估骨质疏松性骨。在这里，我们证明了在骨质疏松症啮齿动物模型中，使用高分辨率X射线成像作为与双能X射线骨密度测定法（DXA）相比可比较的骨变性测量方法的可行性。在第0周，对动物进行卵巢切除术（OVX）或假手术（SHAM）。每周进行DXA分析，以确认和比较OVX引起的骨退行性变化。由于需要去除软组织，因此在尸检后使用高分辨率X射线成像（Faxitron ？）进行了比较。利用了两个感兴趣的区域（ROI）：股骨的远端三分之一和腰椎（L4 / L5）。观察到，与OVX动物相比，SHAM动物保持相对恒定的骨矿物质密度（BMD），从而可以看出BMD的显着降低。进行验尸X射线扫描并将其转换为8位颜色并进行量化。在X射线定量分析中观察到与DXA定量的高度一致性，并且在动物组之间的不透射线（通过颜色分布可视化）与DXA BMD值之间存在明显的相关性。我们的研究证明了定性和定量地高分辨率X射线成像的适用性，作为在啮齿类骨质疏松症模型中量化骨质疏松症的可靠方法。由于DXA是高度依赖用户的模式，因此我们的技术是一种独特的辅助方法，可以验证DXA的发现并最大程度地减少观察者之间的差异。

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