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首页> 外文期刊>BioResearch open access. >Establishment and Characterization of a Singaporean Chinese Lung Adenocarcinoma Cell Line with Four Copies of the Epidermal Growth Factor Receptor Gene
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Establishment and Characterization of a Singaporean Chinese Lung Adenocarcinoma Cell Line with Four Copies of the Epidermal Growth Factor Receptor Gene

机译：表皮生长因子受体基因四份的新加坡华人肺腺癌细胞株的建立和鉴定

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摘要

We have established a lung adenocarcinoma cell line, ETCC016, from lung pleural effusion of a male Singaporean Chinese with advanced lung adenocarcinoma. The subject smoked 20 cigarettes per day for more than 30 years. The cell line arose from spontaneous transformation of cells grown in a collagen-coated culture dish. Transformed characteristics of the cell line include the ability to reach high confluency in a culture dish, low cell doubling time, ability to form colonies in soft agar, and ability to form solid tumor in immune-compromised SCID mice. Immunostaining showed that the cells originated from lung epithelial cells. Genomic analysis revealed a large amount of chromosomal aberrations (gain and loss of genetic materials, and loss of heterozygosity [LOH]), indicative of a long history of smoking. The cells have four copies of epidermal growth factor receptor ( EGFR ) and three copies of MYC , but have lost one copy of the RB1 gene. LOH was detected in TP53 and BRAF genes. There is no anaplastic lymphoma kinase ( ALK ) gene rearrangement. The ETCC016 lung adenocarcinoma cell line has demonstrated susceptibility towards inhibitors specific for EGFR/HER2 and ALK targets, but resistance to MYC-specific inhibitor. This cell line will be a useful model for further understanding of lung adenocarcinoma.
机译：我们已经从一名患有晚期肺腺癌的男性新加坡华人的肺胸腔积液中建立了一个肺腺癌细胞系ETCC016。该对象每天抽20支烟超过30年。该细胞系源自在胶原蛋白包被的培养皿中生长的细胞的自发转化。细胞系的转化特征包括在培养皿中达到高融合度的能力，低细胞倍增时间，在软琼脂中形成菌落的能力以及在免疫受损的SCID小鼠中形成实体瘤的能力。免疫染色显示细胞起源于肺上皮细胞。基因组分析显示大量染色体畸变（遗传物质的获取和损失，以及杂合性[LOH]的损失），表明吸烟历史悠久。该细胞具有四个拷贝的表皮生长因子受体（EGFR）和三个拷贝的MYC，但丢失了一个拷贝的RB1基因。在TP53和BRAF基因中检测到LOH。没有变性间变性淋巴瘤激酶（ALK）基因重排。 ETCC016肺腺癌细胞系已显示出对针对EGFR / HER2和ALK靶标具有特异性的抑制剂的敏感性，但对MYC特异性抑制剂具有抗性。该细胞系将是进一步了解肺腺癌的有用模型。

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