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首页> 外文期刊>BioResearch open access. >CD45+ Cells Present Within Mesenchymal Stem Cell Populations Affect Network Formation of Blood-Derived Endothelial Outgrowth Cells
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CD45+ Cells Present Within Mesenchymal Stem Cell Populations Affect Network Formation of Blood-Derived Endothelial Outgrowth Cells

机译：存在于间充质干细胞群中的CD45 +细胞影响血液来源的内皮生长细胞的网络形成

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Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) represent promising cell sources for angiogenic therapies. There are, however, conflicting reports regarding the ability of MSCs to support network formation of endothelial cells. The goal of this study was to assess the ability of human bone marrow-derived MSCs to support network formation of endothelial outgrowth cells (EOCs) derived from umbilical cord blood EPCs. We hypothesized that upon in vitro coculture, MSCs and EOCs promote a microenvironment conducive for EOC network formation without the addition of angiogenic growth supplements. EOC networks formed by coculture with MSCs underwent regression and cell loss by day 10 with a near 4-fold and 2-fold reduction in branch points and mean segment length, respectively, in comparison with networks formed by coculture vascular smooth muscle cell (SMC) cocultures. EOC network regression in MSC cocultures was not caused by lack of vascular endothelial growth factor (VEGF)-A or changes in TGF-β1 or Ang-2 supernatant concentrations in comparison with SMC cocultures. Removal of CD45+ cells from MSCs improved EOC network formation through a 2-fold increase in total segment length and number of branch points in comparison to unsorted MSCs by day 6. These improvements, however, were not sustained by day 10. CD45 expression in MSC cocultures correlated with EOC network regression with a 5-fold increase between day 6 and day 10 of culture. The addition of supplemental growth factors VEGF, fibroblastic growth factor-2, EGF, hydrocortisone, insulin growth factor-1, ascorbic acid, and heparin to MSC cocultures promoted stable EOC network formation over 2 weeks in vitro , without affecting CD45 expression, as evidenced by a lack of significant differences in total segment length ( p =0.96). These findings demonstrate the ability of MSCs to support EOC network formation correlates with removal of CD45+ cells and improves upon the addition of soluble growth factors.
机译：间充质干细胞（MSC）和内皮祖细胞（EPC）代表了有希望的血管生成疗法的细胞来源。然而，关于MSC支持内皮细胞网络形成的能力的报道相互矛盾。这项研究的目的是评估人类骨髓间充质干细胞支持源自脐带血EPC的内皮生长细胞（EOC）网络形成的能力。我们假设，在体外共培养后，MSC和EOC促进了有利于EOC网络形成的微环境，而无需添加血管生成生长补充剂。与通过共培养血管平滑肌细胞（SMC）形成的网络相比，与MSC共培养形成的EOC网络在第10天经历了回归和细胞丢失，分支点和平均节段长度分别减少了近4倍和2倍。共培养。与SMC共培养相比，MSC共培养中的EOC网络回归不是由缺乏血管内皮生长因子（VEGF）-A或TGF-β1或Ang-2上清液浓度变化引起的。从第6天起，与未分选的MSC相比，从MSC中去除CD45 +细胞可改善EOC网络的形成，与未分选的MSC相比，其总片段长度和分支点数增加了2倍。但是，这些改善在第10天时并未得到维持。共培养与EOC网络回归相关，在培养的第6天到第10天之间增加了5倍。 MSC共培养物中添加补充生长因子VEGF，成纤维细胞生长因子2，EGF，氢化可的松，胰岛素生长因子-1，抗坏血酸和肝素可在体外培养两周内促进稳定的EOC网络形成，而不会影响CD45的表达。总片段长度缺乏显着差异（p = 0.96）。这些发现表明，MSC支持EOC网络形成的能力与CD45 +细胞的去除有关，并在添加可溶性生长因子后得到改善。

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