• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Complementary and Alternative Medicine >Evaluation of antitumor property of extracts and steroidal alkaloids from the cultivated Bulbus Fritillariae ussuriensis and preliminary investigation of its mechanism of action
【24h】

Evaluation of antitumor property of extracts and steroidal alkaloids from the cultivated Bulbus Fritillariae ussuriensis and preliminary investigation of its mechanism of action

机译：栽培的贝母贝母提取物和甾体生物碱的抗肿瘤性质评价及其作用机理的初步研究

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Cancer is well known as a leading cause of death in the world. At present, it is the very active area to search for anticancer drugs from natural products. In this study, we evaluated the antitumor property of chloroform extract (CE), n-hexane extract (HE), water extract (WE) and steroidal alkaloids from the cultivated Bulbus Fritillariae ussuriensis (BFU) and its preliminary mechanism for its action was investigated. Methods Firstly, cytotoxicity of the different extracts from BFU against Lewis lung carcinoma cell line (LLC), Human ovarian cancer cell line (A2780), human hepatocellular carcinoma cell line (HepG2), human lung carcinoma cell line (A549) was measured by MTT assay. Then, we identified the compounds from the active extract of BFU by bioassay guided isolation, determined their antitumor activity in vitro , and detected cell cycle distribution using flow cytometry. Moreover, the extract of BFU which showed remarked anti-proliferative activity in vitro was further evaluated using S180 and LLC tumor models. Additionally, a preliminary investigation of the mechanism of the action was carried out by using histological and immunohistochemical staining technique. Results The results showed that CE and the purified total alkaloids of BFU (TAFU) exhibited stronger cytotoxic activity than the others (WE and HE). We further isolated the four main steroidal alkaloids from TAFU, and found all alkaloids showed significant cytotoxicity, and peimisine induced G0/G1 phase arrest and increased apoptosis. The results showed that TAFU had significant antitumor activity and low toxicity in vivo . Additionally, the immunohistochemical examinations signified that TAFU remarkably increased caspase-3 expression and reduced microvessel density (MVD) in tumor tissues of transplantable S180 and LLC tumor models. Conclusions These results achieved suggested that the steroidal alkaloids could hold a good potential for use as an antitumor drug. Notably, our finding is the first report on the antitumor activity of extracts and steroidal alkaloids from the cultivated BFU in vitro and in vivo and its mechanisms.
机译：背景技术众所周知，癌症是世界上主要的死亡原因。目前，从天然产物中寻找抗癌药物是非常活跃的领域。在这项研究中，我们评估了氯仿提取物（CE），正己烷提取物（HE），水提取物（WE）和栽培的乌苏贝母（BFU）的甾体生物碱的抗肿瘤特性，并研究了其作用的初步机理。 。方法首先用MTT法测定BFU提取物对Lewis肺癌细胞株（LLC），人卵巢癌细胞株（A2780），人肝癌细胞株（HepG2），人肺癌细胞株（A549）的细胞毒性。分析。然后，我们通过生物测定指导分离从BFU活性提取物中鉴定了这些化合物，确定了它们的体外抗肿瘤活性，并使用流式细胞仪检测了细胞周期分布。此外，使用S180和LLC肿瘤模型进一步评估了在体外具有显着抗增殖活性的BFU提取物。另外，通过使用组织学和免疫组织化学染色技术对作用机理进行了初步研究。结果结果表明，CE和纯化的BFU总生物碱（TAFU）显示出比其他（WE和HE）更强的细胞毒活性。我们进一步从TAFU中分离出四种主要甾体生物碱，发现所有生物碱均具有显着的细胞毒性，并且peimisine诱导了G 0 / G 1 的相停并增加了细胞凋亡。结果表明，TAFU在体内具有明显的抗肿瘤活性和低毒性。此外，免疫组织化学检查表明，TAFU在可移植S180和LLC肿瘤模型的肿瘤组织中显着增加了caspase-3表达，并降低了微血管密度（MVD）。结论取得的这些结果表明甾体生物碱具有用作抗肿瘤药的良好潜力。值得注意的是，我们的发现是关于培养的BFU的提取物和甾体生物碱在体内外的抗肿瘤活性及其机理的首次报道。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号