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首页> 外文期刊>BioTechnology: An Indian Journal >Optimization of biomass and pigment production by Penicillium species isolated form virgin forest floor
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Optimization of biomass and pigment production by Penicillium species isolated form virgin forest floor

机译：从原始林地分离青霉菌种优化生物量和色素生产

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摘要

Extracellular pigment-producing ascomycetous filamentous fungi belonging to the genera Penicillium was obtained from soil and its optimal culture conditions investigated. The effect of nutrient culture media, pH and temperature on growth, sporulation and pigment production of three Penicillium species isolated from virgin forest floors of North east India was studied to optimize the mycelial growth and secondary metabolite production. Culture media significantly affected the growth, sporulation and conidial discharge of the species. The average colony growth of the three Penicillium species was higher in Malt Extract Agar (MEA) medium (4.1±0.59cm) whereas spore production was higher in Potato Dextrose Agar (PDA) medium (87.3±3.1). The optimal culture conditions for pigment production were as follows: inoculum???s age, 7 days; temperature, 25 °C; pH, 6 and medium, PDA. Mycelial growth had also a significant correlation with the media pH and incubation temperature together with the pigment production. Under the optimal conditions obtained in the flask culture tested, the isolates secreted coloured pigments into the culture medium which was dependent on the pH of the solution. The high concentration of pigments produced by Penicillium species may be utilized for commercial production of pigments or the metabolites having industrial importance.
机译：从土壤中获得了属于青霉属的产生胞外色素的子囊状丝状真菌，并研究了其最佳培养条件。研究了营养培养基，pH和温度对从印度东北部原始森林地表分离的三种青霉菌的生长，孢子形成和色素生成的影响，以优化菌丝生长和次生代谢产物的产生。培养基极大地影响了该物种的生长，孢子形成和分生孢子。在麦芽提取琼脂（MEA）培养基（4.1±0.59cm）中，三种青霉菌的平均菌落生长较高，而在马铃薯葡萄糖琼脂（PDA）培养基中（87.3±3.1），孢子产生较高。产生色素的最佳培养条件如下：接种物的年龄为7天;温度25°C; pH，6和中等，PDA。菌丝体的生长与培养基的pH，孵育温度以及色素的产生也具有显着的相关性。在测试的烧瓶培养物中获得的最佳条件下，分离物将有色颜料分泌到培养基中，这取决于溶液的pH。由青霉属物种产生的高浓度颜料可用于颜料或具有工业重要性的代谢物的商业生产。

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