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首页> 外文期刊>BMC Biotechnology >Hydrolysis of Agave fourcroydes Lemaire (henequen) leaf juice and fermentation with Kluyveromyces marxianus for ethanol production
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Hydrolysis of Agave fourcroydes Lemaire (henequen) leaf juice and fermentation with Kluyveromyces marxianus for ethanol production

机译：龙舌兰Lyaire（henequen）叶汁的水解和马克斯克鲁维酵母的发酵以生产乙醇

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Background Carbon sources for biofuel production are wide-ranging and their availability depends on the climate and soil conditions of the land where the production chain is located. Henequen ( Agave fourcroydes Lem.) is cultivated in Yucatán, Mexico to produce natural fibers from the leaves, and a juice containing fructans is produced during this process. Fructans can be hydrolyzed to fructose and glucose and metabolized into ethanol by appropriate yeasts. In Mexico, different Agave species provide the carbon source for (distilled and non-distilled) alcoholic beverage production using the stem of the plant, whilst the leaves are discarded. In this work, we investigated the effect of thermal acid and enzymatic hydrolysis of the juice on the amount of reducing sugars released. Growth curves were generated with the yeasts Saccharomyces cerevisiae and Kluyveromyces marxianus and fermentations were then carried out with Kluyveromyces marxianus to determine alcohol yields. Results With thermal acid hydrolysis, the greatest increase in reducing sugars (82.6%) was obtained using 5% H2SO4 at 100°C with a 30?min reaction time. Statistically similar results can be obtained using the same acid concentration at a lower temperature and with a shorter reaction time (60°C, 15?min), or by using 1% H2SO4 at 100°C with a 30?min reaction time. In the case of enzymatic hydrolysis, the use of 5.75, 11.47 and 22.82 U of enzyme did not produce significant differences in the increase in reducing sugars. Although both hydrolysis processes obtained similar results, the difference was observed after fermentation. Ethanol yields were 50.3?±?4 and 80.04?±?5.29% of the theoretical yield respectively. Conclusions Final reducing sugars concentrations obtained with both thermal acid and enzymatic hydrolysis were similar. Saccharomyces cerevisiae , a good ethanol producer, did not grow in the hydrolysates. Only Kluyveromyces marxianus was able to grow in them, giving a higher ethanol yield with the enzymatic hydrolysate. The leaves account for a non-negligible weight of the total agave plant biomass, so this work complements the knowledge already developed on agave fermentations by making it possible to produce ethanol from almost the entire plant (stem and leaves).
机译：背景技术用于生物燃料生产的碳源种类繁多，其可用性取决于生产链所在土地的气候和土壤条件。 Henequen（龙舌兰fourcroydes Lem。）在墨西哥的尤卡坦州种植，从叶片中产生天然纤维，在此过程中产生了含有果聚糖的果汁。果糖聚糖可以水解为果糖和葡萄糖，并通过适当的酵母代谢为乙醇。在墨西哥，不同的龙舌兰物种使用植物的茎为（蒸馏和非蒸馏）酒精饮料生产提供碳源，而叶子则被丢弃。在这项工作中，我们研究了热酸和果汁的酶促水解对释放的还原糖数量的影响。用酵母酿酒酵母和马克斯克鲁维酵母产生生长曲线，然后用马克斯克鲁维酵母进行发酵以确定酒精产量。结果通过热酸水解，在100°C下使用5％H 2 SO 4 在30？min的反应时间下，还原糖的增加最大（82.6％）。 。在较低的温度和较短的反应时间（60°C，15？min）下使用相同的酸浓度，或使用1％H 2 SO 4可获得统计上相似的结果在100°C下反应30分钟。在酶促水解的情况下，使用5.75、11.47和22.82 U的酶对还原糖的增加没有明显的影响。尽管两种水解过程均获得相似的结果，但发酵后观察到差异。乙醇的收率分别为理论收率的50.3％±4和80.04％±5.29％。结论通过热酸和酶水解获得的最终还原糖浓度相似。酿酒酵母，一种良好的乙醇生产商，没有在水解产物中生长。只有马克斯克鲁维酵母（Kluyveromyces marxianus）能够在其中生长，并通过酶促水解产物获得更高的乙醇产量。叶子占龙舌兰植物总生物量的不可忽略的重量，因此这项工作通过使几乎整个植物（茎和叶）产生乙醇成为可能，补充了在龙舌兰发酵中已经开发的知识。

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