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Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips

机译:使用等离激元金芯片上的高灵敏度蛋白阵列检测人血清和滑液中的类风湿因子并同时评估类风湿因子

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Background Fluorescence-enhancing microarray on plasmonic gold film is an attractive alternative to traditional enzyme-linked immunosorbent assay (ELISA) for cytokine detection because of the increased sensitivity. The assay chemistry is similar to an ELISA sandwich assay, but owing to the gold substrate, cytokine measurements are 10 to 100 times more sensitive and can be multiplexed. Plasmonic protein microarrays are, as other immunoassays, affected by the presence of heterophilic antibodies and rheumatoid factor may lead to analytical errors with serious implications for patient care. Here, we present a plasmonic gold substrate protein microarray for high-sensitivity detection of cytokines with simultaneous assessment of rheumatoid factor interference on a single chip.
机译:背景技术由于提高了灵敏度,等离子金膜上的荧光增强微阵列是用于细胞因子检测的传统酶联免疫吸附测定(ELISA)的有吸引力的替代方法。该测定的化学过程类似于ELISA夹心测定,但是由于具有金底物,因此细胞因子的测定灵敏度要高10到100倍,并且可以复用。与其他免疫测定一样,血浆蛋白微阵列也受到异源抗体的影响,类风湿因子可能导致分析错误,对患者的护理产生严重影响。在这里,我们介绍了用于细胞因子的高灵敏度检测与同时评估类风湿因子干扰在单个芯片上的等离激元金底物蛋白质微阵列。

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