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首页> 外文期刊>BioTechnology: An Indian Journal >Effect of Spirulina supplementation on DNA damage after exhaustive exercise in athlete men
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Effect of Spirulina supplementation on DNA damage after exhaustive exercise in athlete men

机译：螺旋藻补充对力竭运动后DNA损伤的影响。

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This study was performed to determine Effect of short-term spirulina supplementation on DNA damage after exhaustive exercise in athlete men. Twenty male athletes (aged 21.06±1.02years, weight 65.12±1.83 kg and height 172.25±1.63 cm and BMI 21.73±0.73 kg/m2) in a randomized and double-blind design were allocated in two equal supplement and placebo groups (3g/day spirulina for 14 days). After supplementation, all participants were participated in bruce test. The blood samples were taken in three phases (before and after the supplementation and after the exercise). This experiment used single cell gel electrophoresis including modified comet assay (with FPG and ENDO III enzymes) to detect the oxidative DNA damage induced by exhaustive exercise in athlete blood cells, and the product 8-hydroxy- 2-deoxyguanosine (8-OHdG) of oxdation of DNA, also, the level of MDA, BUN and CK in athlete plasma were observed. The normal data (Mean ± SD) were analyzed by repeated measure ANOVA, Tukey and independent t-test (P≤0.05). The results showed that a 14-day spirulina supplementation hadn???t significant effect on the damage index of modified comet assay (with FPG and ENDO III enzymes) and the level of 8-OHdG and also the level of MDA, BUN and CK in plasma at rest status (P>0.05). However, exercise-induced decrease of 8-OHdG and the value of comet assay and the plasma level of MDA, BUN and CK in the spirulina group were significantly more in comparison with those in the placebo group (P<0.01). Result of the study indicates that 14-day spirulina supplementation can reduce the production of lipid oxidation and oxidative DNA damage during exhaustive exercise.
机译：进行这项研究以确定短期螺旋藻补充剂对力竭运动的男性运动员DNA损伤的影响。随机和双盲设计的20名男性运动员（年龄21.06±1.02岁，体重65.12±1.83 kg，身高172.25±1.63 cm和BMI 21.73±0.73 kg / m2）被分为两个相等的补充和安慰剂组（3g /螺旋藻（14天）。补充后，所有参与者都参加了布鲁斯测试。在三个阶段中（补充前后，运动之后）采集血样。该实验使用单细胞凝胶电泳，包括改良的彗星试验（使用FPG和ENDO III酶）来检测运动员运动性血细胞中力竭运动引起的氧化DNA损伤，以及产品的8-羟基-2-脱氧鸟苷（8-OHdG）。观察到DNA的氧化，以及运动员血浆中MDA，BUN和CK的水平。通过重复测量方差分析，Tukey和独立t检验分析正常数据（均值±SD）（P≤0.05）。结果表明，补充螺旋藻14天对改良彗星试验（使用FPG和ENDO III酶）的损伤指数，8-OHdG以及MDA，BUN和CK的水平没有显着影响。在血浆中处于静止状态（P> 0.05）。然而，与安慰剂组相比，螺旋藻组运动引起的8-OHdG下降以及彗星试验的价值以及血浆MDA，BUN和CK的水平明显高于安慰剂组（P <0.01）。研究结果表明，补充14天的螺旋藻可以减少力竭运动过程中脂质氧化的产生和DNA的氧化损伤。

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