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首页> 外文期刊>BMC Biotechnology >Enhancement of fermentable sugar yield by competitive adsorption of non-enzymatic substances from yeast and cellulase on lignin
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Enhancement of fermentable sugar yield by competitive adsorption of non-enzymatic substances from yeast and cellulase on lignin

机译：通过竞争性地将来自酵母和纤维素酶的非酶物质吸附在木质素上来提高可发酵糖的产量

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Background Enhancement of enzymatic digestibility by some supplementations could reduce enzyme loading and cost, which is still too high to realize economical production of lignocellulosic biofuels. A recent study indicates that yeast hydrolysates (YH) have improved the efficiency of cellulases on digestibility of furfural residues (FR). In the current work, the components of YH were separated by centrifugation and size exclusion chromatography and finally characterized in order to better understand this positive effect. Results A 60.8% of nitrogen of yeast cells was remained in the slurry (YHS) after hydrothermal treatment. In the supernatant of YH (YHL), substances of high molecular weight were identified as proteins and other UV-absorbing compounds, which showed close molecular weight to components of cellulases. Those substances attributed to a synergetic positive effect on enzymatic hydrolysis of FR. The fraction of YHL ranged from 1.19 to 2.19?mL (elution volume) contained over 50% of proteins in YHL and had the best performance in stimulating the release of glucose. Experiment results proved the adsorption of proteins in YHL on lignin. Conclusions Supplementation of cellulases with YH enhances enzymatic digestibility of FR mainly by a competitive adsorption of non-enzymatic substances on lignin. The molecular weight of these substances has a significant impact on their performance. Different strategies can be used for a good utilization of yeast cells in terms of biorefinery concept.
机译：背景技术通过一些补充提高酶消化率可以降低酶的负载和成本，这对于实现经济生产木质纤维素生物燃料而言仍然太高。最近的一项研究表明，酵母水解物（YH）改善了纤维素酶对糠醛残渣（FR）消化率的效率。在目前的工作中，通过离心和尺寸排阻色谱法分离了YH的成分，并对其进行了最终表征，以更好地了解这种积极作用。结果水热处理后，浆液（YHS）中酵母细胞的氮含量为60.8％。在YH（YHL）的上清液中，高分子量物质被鉴定为蛋白质和其他吸收紫外线的化合物，其分子量与纤维素酶的成分接近。这些物质归因于对FR的酶促水解的协同正作用。 YHL的含量范围为1.19至2.19？mL（洗脱体积），其中YHL中的蛋白质含量超过50％，并且在刺激葡萄糖释放方面表现最佳。实验结果证明了YHL中的蛋白质在木质素上的吸附。结论纤维素酶补充YH主要通过非酶物质在木质素上的竞争性吸附来提高FR的酶消化率。这些物质的分子量对其性能有重大影响。就生物精炼概念而言，可以采用不同的策略来更好地利用酵母细胞。

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