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Biocatalytic potential of vanillin aminotransferase from Capsicum chinense

机译：辣椒中香兰素转氨酶的生物催化潜力

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Background The conversion of vanillin to vanillylamine is a key step in the biosynthetic route towards capsaicinoids in pungent cultivars of Capsicum sp. The reaction has previously been annotated to be catalysed by PAMT (putative aminotransferase; [GenBank: AAC78480.1, Swiss-Prot: O82521]), however, the enzyme has previously not been biochemically characterised in vitro . Results The biochemical activity of the transaminase was confirmed by direct measurement of the reaction with purified recombinant enzyme. The enzyme accepted pyruvate, and oxaloacetate but not 2-oxoglutarate as co-substrate, which is in accordance with other characterised transaminases from the plant kingdom. The enzyme was also able to convert ( S )-1-phenylethylamine into acetophenone with high stereo-selectivity. Additionally, it was shown to be active at a broad pH range. Conclusions We suggest PAMT to be renamed to VAMT (vanillin aminotransferase, abbreviation used in this study) as formation of vanillin from vanillylamine could be demonstrated. Furthermore, due to high stereoselectivity and activity at physiological pH, VAMT is a suitable candidate for biocatalytic transamination in a recombinant whole-cell system.
机译：背景技术香兰素向香兰素胺的转化是辣椒辛辣型辛辣品种朝辣椒素生物合成途径中的关键步骤。该反应先前已被注释为被PAMT催化（公认的氨基转移酶; [GenBank：AAC78480.1，Swiss-Prot：O82521]），但是，该酶先前尚未在体外进行生化表征。结果转氨酶的生化活性通过直接测定纯化的重组酶的反应而得到证实。该酶接受丙酮酸和草酰乙酸而不是2-氧代戊二酸作为共底物，这与来自植物界的其他特征性转氨酶一致。该酶还能够以高的立体选择性将（S）-1-苯基乙胺转化为苯乙酮。此外，它在广泛的pH范围内具有活性。结论我们建议将PAMT重命名为VAMT（香草醛氨基转移酶，本研究中使用的缩写），因为可以证明由香草醛胺形成了香草醛。此外，由于高的立体选择性和在生理pH值下的活性，VAMT是重组全细胞系统中生物催化转氨作用的合适候选者。

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