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首页> 外文期刊>BMC Veterinary Research >A high-concentrate diet induced colonic epithelial barrier disruption is associated with the activating of cell apoptosis in lactating goats
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A high-concentrate diet induced colonic epithelial barrier disruption is associated with the activating of cell apoptosis in lactating goats

机译：高浓度饮食诱导的结肠上皮屏障破坏与泌乳山羊细胞凋亡的激活有关

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Background In ruminants, lower ruminal pH causes massive disruption of ruminal epithelial structure during periods of feeding high-concentrate diets. However, the influence of excessive organic fatty acids in the lumen of hindgut on the epithelial structure is unclear. In this study, twelve mid-lactating goats were randomly assigned to either a HC diet group (65% concentrate of dry matter; n = 6) or a LC diet group (35% concentrate of dry matter; n = 6) for 10 weeks. The colonic epithelial structure was detected by HE staining and transmission electron microscopy (TEM), and the apoptotic status of epithelial cells was estimated by TUNEL method and caspase activities. Results HC goats showed higher level of free lipopolysaccharide (LPS) in rumen fluid ( p??0.05 ), and higher total volatile fatty acid (VFA) concentrations in rumen fluid ( p??0.05 ). Conclusions Our results reveal that long-term feeding HC diet to lactating goats causes severe damages to the colonic mucosa barrier associated with activating cells apoptosis.
机译：背景技术在反刍动物中，较低的瘤胃pH值会导致高浓度饮食喂养期间瘤胃上皮结构的大量破坏。但是，尚不清楚后肠腔中过量的有机脂肪酸对上皮结构的影响。在这项研究中，将十二只中哺乳期山羊随机分为HC饮食组（65％干物质; n = 6）或LC饮食组（35％干物质浓度; n = 6），持续10周。 HE染色和透射电镜（TEM）检测结肠上皮结构，TUNEL法和胱天蛋白酶活性评估上皮细胞的凋亡状态。结果HC山羊瘤胃液中游离脂多糖（LPS）含量较高（p ?? 0.05），瘤胃液中总挥发性脂肪酸（VFA）含量较高（p ?? 0.05）。结论我们的结果表明，长期向哺乳的山羊喂饲HC饮食会严重破坏与激活细胞凋亡相关的结肠粘膜屏障。

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