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首页> 外文期刊>BMC Veterinary Research >Isolation and characterization of porcine epidemic diarrhea virus associated with the 2014 disease outbreak in Mexico: case report
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Isolation and characterization of porcine epidemic diarrhea virus associated with the 2014 disease outbreak in Mexico: case report

机译：与2014年墨西哥疾病暴发有关的猪流行性腹泻病毒的分离和鉴定：病例报告

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Background Interest in porcine epidemic diarrhea has grown since the 2013 outbreak in the United States caused major losses, with mortality rates up to 100?% in suckling piglets. In Mexico, an outbreak of porcine epidemic diarrhea, characterized by 100?% mortality in piglets, began in March 2014 in the State of Mexico. Methods The aim of this study was to confirm and identify porcine epidemic diarrhea virus (PEDV) in samples from piglets with suggestive clinical signs using virological, histological, and molecular techniques. Necropsy was performed on 13 piglets from two litters with initial and advanced clinical signs. Suggestive lesions of acute infection with PEDV were detected in histological sections of the small and large bowels; specifically, multiple virus particles with visible crown-shaped projections were observed using electron microscopy and negative staining. Viral isolation was performed in Vero cells with trypsin. Infection was monitored by observation of cytopathic effect, and titration was determined by TCID50/ml. The presence of the PEDV in cultures and clinical samples was confirmed by RT-PCR amplification and sequencing of a 651-bp segment of the S glycoprotein gene, as well as a 681-bp matrix protein gene. Results The nucleotide sequence analysis of the Mexican isolates showed marked homology to viruses that circulated in 2013 in Colorado, USA. Conclusions In this paper we confirm the isolation and characterization of PEDV from animals with early and advanced clinical signs.
机译：背景自从2013年美国爆发禽流感引起重大损失以来，人们对猪流行性腹泻的兴趣不断增长，乳猪的死亡率高达100％。墨西哥于2014年3月开始爆发猪流行性腹泻，其特点是仔猪死亡率达到100％。方法本研究的目的是使用病毒学，组织学和分子技术，对具有提示性临床症状的仔猪样本中的猪流行性腹泻病毒（PEDV）进行确认和鉴定。对来自两个窝的13头仔猪进行尸检，这些仔猪具有初始和晚期临床症状。在小肠和大肠的组织切片中发现了PEDV急性感染的提示性病变。具体而言，使用电子显微镜和阴性染色观察到多个具有可见冠状突起的病毒颗粒。用胰蛋白酶在Vero细胞中进行病毒分离。通过观察细胞病变作用监测感染，并通过TCID 50 / ml测定滴定度。通过RT-PCR扩增和测序S糖蛋白基因的651 bp片段以及681 bp的基质蛋白基因，证实了培养物和临床样品中PEDV的存在。结果墨西哥分离物的核苷酸序列分析显示与2013年在美国科罗拉多州传播的病毒具有明显的同源性。结论在本文中，我们确认了从具有早期和晚期临床体征的动物中分离并鉴定了PEDV的特征。

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