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首页> 外文期刊>Biotropia: the Southeast Asian journal of tropical biology >SOMATIC EMBRYOGENESIS FROM MERISTEM EXPLANTS OF GINGER
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SOMATIC EMBRYOGENESIS FROM MERISTEM EXPLANTS OF GINGER

机译：从姜的侵犯部位的体细胞胚发生

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Normal 0 false false false MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable{mso-style-name:"Table Normal";mso-tstyle-rowband-size:0;mso-tstyle-colband-size:0;mso-style-noshow:yes;mso-style-parent:"";mso-padding-alt:0mm 5.4pt 0mm 5.4pt;mso-para-margin:0mm;mso-para-margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:10.0pt;font-family:"Times New Roman";mso-ansi-language:#0400;mso-fareast-language:#0400;mso-bidi-language:#0400;} The use of planting materials from in vitro culture, especially derived from somatic embryos has some advantages such as ? genetically stable and pathogen-free. Meristem culture of ginger through somatic embryogenesis could be a potential method for producing pathogen-free planting materials. Somatic embryogenesis on ginger was performed to obtain vigorous plantlets having the same rhizome size as the mother plant. Callus was induced from meristem tissue of inner bud of Indonesian ginger rhizome Var. Cimanggu-1 and consecutively subcultured into certain media at each steps of experiments. The vigorous embryogenic calli were observed ? on MS medium containing 100 mgl -1 glutamine and 2% sucrose with addition of 1.0 mgl -1 2,4-D + 3.0 mgl -1 BA. The highest number of somatic embryos (about 82.0.g -1 friable calli) was achieved on that medium, 4 weeks after culturing. Furthermore, the optimum growth of embryogenic calli containing somatic embryo was obtained on MS medium enriched with 6% sucrose. The highest number of mature somatic embryos (57.2 embryos) was achieved on MS medium, 18 days after incubation. The regeneration potency of somatic embryos obtained from ginger meristem was 51.20%.g -1 friable callus. The valuable result of this study was the achievement of normal rhizome size of regenerated plantlets, instead of micro rhizome.
机译：正常0否否否MicrosoftInternetExplorer4 / *样式定义* / table.MsoNormalTable {mso-style-name：“ Table Normal”; mso-tstyle-rowband-size：0; mso-tstyle-colband-size：0; mso-style -noshow：是; mso-style-parent：“”; mso-padding-alt：0mm 5.4pt 0mm 5.4pt; mso-para-margin：0mm; mso-para-margin-bottom：.0001pt; mso-pagination：寡妇孤儿;字体大小：10.0pt;字体家族：“ Times New Roman”; mso-ansi语言：＃0400; mso-fareast语言：＃0400; mso-bidi语言：＃0400;}使用体外培养的植物材料，尤其是来自体细胞胚的植物材料具有一些优势，例如？遗传稳定且无病原体。通过体细胞胚发生进行生姜的分生组织培养可能是生产无病原体种植材料的潜在方法。在生姜上进行体细胞胚发生以获得具有与母本植物相同的根茎大小的有活力的小植物。愈伤组织是由印尼姜根茎变种内芽的分生组织诱导的。在实验的每个步骤中，将Cimanggu-1并连续传代到某些培养基中。观察到剧烈的胚性愈伤组织？在含有100 mgl -1谷氨酰胺和2％蔗糖的MS培养基上加入1.0 mgl -1 2,4-D + 3.0 mgl -1 BA。培养4周后，在该培养基上获得了最高数量的体细胞胚（约82.0.g -1脆性愈伤组织）。此外，在富含6％蔗糖的MS培养基上获得了含有胚性愈伤组织的胚性愈伤组织的最佳生长。孵育18天后，在MS培养基上获得了最高数量的成熟体细胞胚（57.2个胚）。姜分生组织得到的体细胞胚再生能力为51.20％.g -1脆性愈伤组织。这项研究的有价值的结果是达到了再生植株的正常根茎大小，而不是微型根茎。

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