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首页> 外文期刊>Brazilian Journal of Medical and Biological Research >Regulation of the expression of NADP-malic enzyme by UV-B, red and far-red light in maize seedlings
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Regulation of the expression of NADP-malic enzyme by UV-B, red and far-red light in maize seedlings

机译：UV-B，红光和远红光对玉米幼苗中NADP-苹果酸酶表达的调控

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摘要

The induction of nicotinamide adenine dinucleotide phosphate-malic enzyme (NADP-ME) in etiolated maize (Zea mays) seedlings by UV-B and UV-A radiation, and different levels of photosynthetically active radiation (PAR, 400-700 nm) was investigated by measuring changes in activity, protein quantity and RNA levels as a function of intensity and duration of exposure to the different radiations. Under low levels of PAR, exposure to UV-B radiation but not UV-A radiation for 6 to 24 h caused a marked increase in the enzyme levels similar to that observed under high PAR in the absence of UV-B. UV-B treatment of green leaves following a 12-h dark period also caused an increase in NADP-ME expression. Exposure to UV-B radiation for only 5 min resulted in a rapid increase of the enzyme, followed by a more gradual rise with longer exposure up to 6 h. Low levels of red light for 5 min or 6 h were also effective in inducing NADP-ME activity equivalent to that obtained with UV-B radiation. A 5-min exposure to far-red light following UV-B or red light treatment reversed the induction of NADP-ME, and this effect could be eliminated by further treatment with UV-B or red light. These results indicate that physiological levels of UV-B radiation can have a positive effect on the induction of this photosynthetic enzyme. The reducing power and pyruvate generated by the activity of NADP-ME may be used for respiration, in cellular repair processes and as substrates for fatty acid synthesis required for membrane repair.
机译：通过UV-B和UV-A辐射在黄化玉米（Zea mays）幼苗中诱导烟酰胺腺嘌呤二核苷酸磷酸苹果酸酶（NADP-ME）的诱导，并研究了不同水平的光合活性辐射（PAR，400-700 nm）通过测量活性，蛋白质数量和RNA水平随强度和暴露于不同辐射的持续时间的变化而变化。在低水平的PAR下，暴露于UV-B辐射但不暴露于UV-A辐射6至24小时，导致酶水平显着增加，类似于在不存在UV-B的高PAR下观察到的酶水平。在黑暗期12小时之后，对绿叶进行UV-B处理也导致NADP-ME表达增加。暴露于UV-B辐射仅5分钟导致酶迅速增加，随后逐渐逐渐增加，暴露时间延长至6小时。持续5分钟或6小时的低水平红光也能有效诱导NADP-ME活性，其活性与UV-B辐射相当。 UV-B或红光处理后，在远红光下暴露5分钟可以逆转NADP-ME的诱导，而进一步用UV-B或红光处理可以消除这种影响。这些结果表明，UV-B辐射的生理水平可以对该光合作用酶的诱导产生积极影响。由NADP-ME的活性产生的还原能力和丙酮酸可用于呼吸，细胞修复过程中以及用作膜修复所需脂肪酸合成的底物。

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