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首页> 外文期刊>Brazilian Journal of Medical and Biological Research >Influence of melatonin on the development of functional nicotinic acetylcholine receptors in cultured chick retinal cells
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Influence of melatonin on the development of functional nicotinic acetylcholine receptors in cultured chick retinal cells

机译：褪黑素对培养的雏鸡视网膜细胞功能性烟碱型乙酰胆碱受体发育的影响

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The influence of melatonin on the developmental pattern of functional nicotinic acetylcholine receptors was investigated in embryonic 8-day-old chick retinal cells in culture. The functional response to acetylcholine was measured in cultured retina cells by microphysiometry. The maximal functional response to acetylcholine increased 2.7 times between the 4th and 5th day in vitro (DIV4, DIV5), while the Bmax value for [125I]-alpha-bungarotoxin was reduced. Despite the presence of alpha8-like immunoreactivity at DIV4, functional responses mediated by alpha-bungarotoxin-sensitive nicotinic acetylcholine receptors were observed only at DIV5. Mecamylamine (100 μM) was essentially without effect at DIV4 and DIV5, while dihydro-?-erythroidine (10-100 μM) blocked the response to acetylcholine (3.0 nM-2.0 μM) only at DIV4, with no effect at DIV5. Inhibition of melatonin receptors with the antagonist luzindole, or melatonin synthesis by stimulation of D4 dopamine receptors blocked the appearance of the alpha-bungarotoxin-sensitive response at DIV5. Therefore, alpha-bungarotoxin-sensitive receptors were expressed in retinal cells as early as at DIV4, but they reacted to acetylcholine only after DIV5. The development of an alpha-bungarotoxin-sensitive response is dependent on the production of melatonin by the retinal culture. Melatonin, which is produced in a tonic manner by this culture, and is a key hormone in the temporal organization of vertebrates, also potentiates responses mediated by alpha-bungarotoxin-sensitive receptors in rat vas deferens and cerebellum. This common pattern of action on different cell models that express alpha-bungarotoxin-sensitive receptors probably reflects a more general mechanism of regulation of these receptors.
机译：在培养的胚胎8日龄雏鸡视网膜细胞中研究了褪黑激素对功能性烟碱型乙酰胆碱受体发育模式的影响。通过显微生理学测量在培养的视网膜细胞中对乙酰胆碱的功能应答。在体外第4天和第5天之间，对乙酰胆碱的最大功能反应增加了2.7倍（DIV4，DIV5），而[125I]-α-真菌毒素的Bmax值却降低了。尽管在DIV4处存在alpha8样免疫反应性，但仅在DIV5处观察到了由α-真菌毒素敏感性烟碱型乙酰胆碱受体介导的功能反应。甲胺基胺（100μM）对DIV4和DIV5基本上没有作用，而二氢-β-赤藓类碱（10-100μM）仅在DIV4时阻止了对乙酰胆碱（3.0 nM-2.0μM）的反应，而对DIV5没有影响。用拮抗剂luzindole抑制褪黑激素受体，或通过刺激D4多巴胺受体来合成褪黑激素，可阻止DIV5出现的α-真菌毒素敏感性反应。因此，早在DIV4时，视网膜细胞中就表达了α-真菌毒素敏感受体，但它们仅在DIV5后才与乙酰胆碱反应。对α-真菌毒素敏感反应的发展取决于视网膜培养物中褪黑激素的产生。褪黑素是这种培养物以补品的方式产生的，并且是脊椎动物暂时组织中的关键激素，它还能增强大鼠输精管和小脑中由α-真菌毒素敏感受体介导的应答。在表达α-真菌毒素敏感受体的不同细胞模型上的这种共同作用模式可能反映了调节这些受体的更一般的机制。

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