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Characterization of Solid State Fermentation Culture Conditions for Growth and Mananase Production by Aspergillus niger USM F4 on Rice Husk in Tray System

机译:塔盘系统中黑曲霉USM F4在固态发酵培养条件下生长和产生甘露聚糖酶的特性

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Aim: The study evaluated various fermentation conditions for the production of mannanase. Place and Duration of Study: Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia between May 2009 and September 2010. Methodology: Solid substrate fermentation was carried out in a shallow aluminum tray system (16 cm x 16 cm x 5 cm) for maximum mannanase production by Aspergillus niger USM F4 using rice husk as a substrate. Results: The maximum mannanase activity of 119.91 U/g substrate was achieved on the 6 days of cultivation when the optimized physical parameters were used (substrate thickness of 1.6 cm or equivalent to 80 g of 0.75 mm rice husk, moisture content to substrate ratio of 1:1 (w/v), cultivation temperature at room temperature (28±2oC), inoculum size of 6x106 spores/ml and in static condition (no mixing during the fermentation process). The results showed an increment of about 30.79% of mannanase activity after the optimization (119.91 U/g substrate) compared to before optimization (91.68 U/g substrate). Conclusion: The results obtained from this study revealed that rice husk can be used as a substrate for mannanase production in solid state fermentation process.
机译:目的:该研究评估了各种发酵条件下甘露聚糖酶的产生。研究的地点和时间:2009年5月至2010年9月,马来西亚槟城11800年,马来西亚大学生物科学学院工业生物技术研究实验室(IBRL)。方法:在浅铝托盘系统中进行固体底物发酵(16 (cm x 16 cm x 5 cm),以黑曲霉USM F4以稻壳为底物最大程度地生产甘露聚糖酶。结果:使用优化的物理参数(底物厚度为1.6 cm或相当于80 g 0.75 mm的稻壳,水分含量与底物比为0.8)时,在优化的物理参数下培养6天,最大甘露聚糖酶活性达到119.91 U / g底物。 1:1(w / v),室温下培养温度(28±2oC),接种量为6x106孢子/ ml,并且在静态条件下(发酵过程中不混合),结果表明发酵液的增量约为30.79%。优化后的甘露聚糖酶活性(119.91 U / g底物)与优化前的甘露聚糖酶的活性(91.68 U / g底物)结论:本研究结果表明稻壳可作为固态发酵过程中生产甘露聚糖酶的底物。

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