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Evaluation of Immunochromatography for the Rapid and Specific Identification of Listeria monocytogenes from Food

机译：免疫色谱法快速，特异性鉴定食品中单核细胞增生李斯特菌的评估

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摘要

To rapidly, simply and specifically detect and identify Listeria monocytogenes from food samples, an immunochromatographic assay, based on gold-labeled monoclonal antibodies directed against an antigen common to all serovars of L. monocytogenes , was used. All strains of L. monocytogenes serovars showed a positive reaction to the assay, but all other gram positive and negative bacteria did not. The detection limit of the assay was in the order of 10~(6) cfu/ml in fluid medium. The assay could simply and rapidly identify L. monocytogenes within 30 min by a pure culture without special instruments. Even if the selective enrichment cultivation was employed for the isolation and growth of bacteria from food materials, the application of the assay system could detect and identify L. monocytogenes precisely in various food materials within 2 to 3 days.
机译：为了快速，简单和特异性地从食品样品中检测和鉴定单核细胞增生李斯特氏菌，基于金标记的单克隆抗体的免疫色谱分析方法，该单克隆抗体针对的是所有血清型共同抗原。使用了单核细胞增生因子。 L所有菌株。单核细胞增生病血清对试验显示阳性反应，但所有其他革兰氏阳性和阴性细菌均无反应。在液体培养基中，该检测的检出限为10〜（6）cfu / ml。该测定法可以简单快速地鉴定出。无需特殊仪器，即可在30分钟内通过纯培养获得单核细胞增生李斯特菌。即使采用选择性富集培养从食品原料中分离和生长细菌，该测定系统的应用也可以检测和鉴定L。在2到3天内精确地在各种食品原料中检测出单核细胞增生病。

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