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首页> 外文期刊>British Biotechnology Journal >Cellulase Producing Potential of Aspergillus terreusUv2 on Cellulosic Wastes Pretreated with Acidand Alkali
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Cellulase Producing Potential of Aspergillus terreusUv2 on Cellulosic Wastes Pretreated with Acidand Alkali

机译：TerreusUv2纤维素酶对酸和碱预处理的纤维素废料的生产潜力

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Aims: Cellulases offer very wide applications in biotechnology and enzymes from microbial origins present inexpensive source. Production of value added chemicals from wastes will be an exciting translation from waste to wealth and an eco-friendly initiative instead of the incineration option often given to cellulosic wastes.Study Design: Sulphuric acid and Sodium hydroxide solutions were prepared at 0.5 M and 2 M concentrations to pretreat three cellulosic wastes that had been made neutral prior to fermentation with a known cellulase producing moldPlace and Duration of Study: All experiments were conducted in the laboratory of the Department of Microbiology, Federal University of Technology, Minna, Nigeria for a period of six weeks.Methodology: Hypercellulase producing Aspergillus terreus UV2 strain was used to ferment pretreated cellulosic wastes: Corn cob, corn straw and bagasse, using submerged fermentation in Mandel basal medium. The crystalline lignocelluloses were milled and fractionated into 850 μ particle size and pretreated in two concentrations (0.5 M and 2 M) of both acid (sulphuric acid) and alkali (sodium hydroxide) independently and were left for varying residence time of one hour or three hours in the digester at ambient temperature, Optimum spore concentration of 1.0 x 106 spores/ml and pH of 4.8. Supernatants of crude enzyme were taken and assayed at 24 hours interval.Results: Cellulase activity peaked at 96 hours. Enzyme secretion in the cellulosic wastes was highest in sugarcane bagasse, followed by the corn cob and then the corn straw corresponding to 51%, 40% and 16% respectively. Alkali pretreated cellulosics gave higher yield of cellulase than its counterpart acid. Non-pretreated residues gave only low enzyme titers. Bagasse produced optimum cellulase yield of 0.068 IU/ml/min within 120 hours when subjected to 2 M NaOH digestion for one hour before fermentation. This translated to 39% increase in enzyme expression when compared with non-treated bagasse of 0.049 IU/ml/min.Conclusion: Sugarcane bagasse therefore when digested with mild alkali (2 M NaOH) for a pretreatment period of one hour holds a great possibility for cellulase production using a mutant mold, Aspergillus terreus UV2. Production of value added chemicals from cellulosic wastes will be an exciting translation from waste to wealth.
机译：目的：纤维素酶在生物技术中应用非常广泛，而微生物来源的酶是目前廉价的来源。从废物中生产增值化学品将是从废物到财富的激动人心的转变，并且是一种环保倡议，而不是通常针对纤维素废物的焚化方案。研究设计：分别以0.5 M和2 M制备硫酸和氢氧化钠溶液浓度和预处理时间：研究的持续时间和时间：所有实验均在尼日利亚明纳联邦技术大学微生物学系的实验室进行，时间为六周。方法：使用产生超纤维素酶的土壤曲霉Aspergillus UV2菌株，通过在Mandel基础培养基中进行深层发酵，发酵预处理的纤维素废料：玉米芯，玉米秸秆和蔗渣。将结晶的木质纤维素研磨并分馏成850μ粒度，并分别在两种浓度（0.5 M和2 M）的酸（硫酸）和碱（氢氧化钠）中进行预处理，并保留一小时或三小时的不同停留时间在室温下在蒸煮器中放置数小时，最佳孢子浓度为1.0 x 106孢子/ ml，pH为4.8。取上清液中的酶上清液，每隔24小时检测一次。结果：纤维素酶活性在96小时达到峰值。纤维素废料中的酶分泌最高的是甘蔗渣，其次是玉米芯，然后是玉米秸秆，分别占51％，40％和16％。碱预处理的纤维素酶比其对应的酸具有更高的纤维素酶收率。未经预处理的残留物仅产生较低的酶滴度。在发酵前进行2 M NaOH消化一小时后，在120小时内，蔗渣产生的纤维素酶最佳产量为0.068 IU / ml / min。与未处理的甘蔗渣（0.049 IU / ml / min）相比，这意味着酶表达增加了39％。结论：因此，甘蔗渣在用弱碱（2 M NaOH）消化一小时的预处理时具有很大的优势。使用突变霉菌曲霉曲霉UV2生产纤维素酶的可能性。用纤维素废料生产增值化学品将是从废料到财富的激动人心的转变。

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