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首页> 外文期刊>British Biotechnology Journal >Investigation of Genetic Diversity among Medicagospecies Using RAPD Markers
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Investigation of Genetic Diversity among Medicagospecies Using RAPD Markers

机译：利用RAPD标记研究杂种的遗传多样性。

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Aims: Medicago is known as the Queen of forage with potential economic importance to our society. The present study aimed at the use of RAPD-PCR DNA marker to identify the genetic fingerprints affinities of six species of Alfalfa.Place and Duration of Study: The study was conducted at the Department of Genetics, Garden Campus, Hazara University, Mansehra Pakistan during February, 2011 to August, 2013.Methodology: In this study, six species of Medicago namely TWAL (Tetraploid Wisconsin Alfalfa Line), Medicago arborea, Medicago falcata, Medicago sativa, Medicago lupulina and Medicago polymorpha were used to explore the diversity of alfalfa. Seven out of 120 decamers produced 34 polymorphic loci with 100% polymorphism to identify the different species of Medicago crop. The range of polymorphic loci was observed from 300 to 700 bp. Eleven species specific loci were generated by seven decamers. Primer B-18 generated single specific locus 700 bp against genomic DNA of M. lupulina and it is important to identify particular species of Alfalfa. The bivariate data were recorded as the presence of locus 1 and absence 0 and then this data was transferred into A and C respectively to make it suitable for DNAMAN software (version 5.2.2.0; Applied Biostatistics Inc). Moreover, cluster analysis was performed using sequence alignment and divergence function of the DNAMAN against the bivariate data collected from the products of decamers. All members clustered in a unique pattern except M. falcata and M. lupulina those shared 86% homology. Three distinct groups were observed during UPGMA (Unweighted pair Group Method with Arithmetic Mean). During the phylogenetic study, TWAL was observed to have genetic diversity from other five species of Alfalfa.Conclusion: So, the present study is enabling us to discriminate different species of Alfalfa and it could be useful to identify and authenticate different species of the same genus of medicinal important plant from the Flora of Pakistan.
机译：目的：Medicago被称为草料女王，对我们的社会具有潜在的经济重要性。本研究旨在使用RAPD-PCR DNA标记物鉴定6种苜蓿的遗传指纹亲和力。研究地点和持续时间：该研究在巴基斯坦曼塞拉哈扎拉大学花园校区遗传学系进行。 2011年2月至2013年8月。方法：在本研究中，使用六种紫花苜蓿，即TWAL（四倍体威斯康星州紫花苜蓿品系），紫花苜蓿，紫花苜蓿，紫花苜蓿，紫花苜蓿和紫花苜蓿来研究紫花苜蓿的多样性。在120个欺骗者中，有7个产生了34个具有100％多态性的多态性位点，以鉴定紫花苜蓿作物的不同物种。观察到多态位点的范围为300至700bp。七个decamer生成了11个物种特异性位点。 B-18引物针对棉铃虫的基因组DNA产生了700 bp的单个特异性位点，因此识别特定的苜蓿物种非常重要。将双变量数据记录为基因座1的存在和不存在0，然后将该数据分别转移到A和C中以使其适用于DNAMAN软件（5.2.2.0版; Applied Biostatistics Inc）。此外，使用DNAMAN的序列比对和发散函数针对从十聚体产物收集的双变量数据进行聚类分析。除f.cata和M. lupulina具有相同的86％同源性外，所有成员均以独特的模式聚集。在UPGMA（使用算术平均值的非加权对分组方法）期间观察到三个不同的组。在系统发育研究中，观察到TWAL具有其他5种苜蓿的遗传多样性。结论：因此，本研究使我们能够区分不同种类的苜蓿，并且可能有助于鉴定和鉴定同一属的不同物种。巴基斯坦植物区系的重要药用植物。

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