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首页> 外文期刊>British Biotechnology Journal >Microsatellite DNA Marker Analysis Revealed LowLevels of Genetic Variability in the Wild and CaptivePopulations of Cirrhinus cirrhosus (Hamilton)(Cyprinidae: Cypriniformes)
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Microsatellite DNA Marker Analysis Revealed LowLevels of Genetic Variability in the Wild and CaptivePopulations of Cirrhinus cirrhosus (Hamilton)(Cyprinidae: Cypriniformes)

机译：微卫星DNA标记分析揭示了野生型和圈养型肝硬化（汉密尔顿）种群的低水平遗传变异（鲤科：鲤形目）

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Aims: To reveal the genetic variability of wild and captive populations of the Indian major carp, mrigal (Cirrhinus cirrhosus) based on microsatellite DNA markers analysis.Study Design: Three rivers namely the Halda, the Padma and the Jamuna were selected under wild population category and three hatcheries such as Brahmaputra Hatchery of Mymensingh, Raipur Government Hatchery, Luxmipur, and Sonali Hatchery, Jessore were selected under the captive population category. Place and Duration of the Study: Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh from July 2005 to June 2008.Methodology: DNA was extracted from fin clips of a total of 180 fish, 30 from each of the six populations. Five microsatellite markers MFW1, MFW2, MFW17, Barb54 and Bgon22 were amplified by polymerase chain reaction for each DNA sample and resolved on denatured polyacrylamide gel and visualized by silver nitrate staining. Results: Three of the five loci were found to be polymorphic in all the six populations. The observed (HO) and expected heterozygosity (HE) ranged from 0.233 to 0.633 and 0.406 to 0.664 respectively. The FIS values ranged from 0.032 to 0.635 indicating deficiency in heterozygosity. Except the Raipur Hatchery stock, the other five populations showed nonconformity to Hardy-Weinberg Expectation at least in one locus. Significant population differentiation was observed between the Halda-Jamuna, Jamuna-Brahmaputra Hatchery, Jamuna-Raipur Hatchery and the Padma-Raipur Hatchery population pairs. The UPGMA dendrogram based on genetic distances resulted in two major clusters: the Halda river and the Raipur Hatchery population were in one cluster and the remaining four populations were in the other cluster.Conclusion: The study, as a whole, revealed low levels of genetic variation in terms of allelic richness and heterozygosity in the three major rivers and three selected hatchery stocks of C. cirrhosus in Bangladesh.
机译：目的：基于微卫星DNA标记分析，揭示印度主要鲤鱼（Cirrhinus cirrhosus）野生和圈养种群的遗传变异性。研究设计：在野生种群类别下选择了三条河流，即霍尔达河，帕德玛河和贾穆纳河并在圈养人口类别中选择了三个孵化场，如迈门辛格的布拉马普特拉孵化场，莱克斯米布尔的赖布尔政府孵化场和杰索尔的索纳里孵化场。研究的地点和持续时间：2005年7月至2008年6月，孟加拉国农业大学渔业生物学与遗传学系，迈门辛。方法：从总共180条鱼的鳍夹中提取DNA，六个种群中的每条鱼有30条。通过DNA聚合酶链反应扩增五个微卫星标记MFW1，MFW2，MFW17，Barb54和Bgon22，并在变性聚丙烯酰胺凝胶上分离，并通过硝酸银染色可视化。结果：在所有六个种群中，五个基因座中的三个被发现具有多态性。观察到的（HO）和预期的杂合度（HE）分别为0.233至0.633和0.406至0.664。 FIS值介于0.032至0.635之间，表明杂合性不足。除了Raipur孵化场的种群外，其他五个种群至少在一个地方显示出不符合Hardy-Weinberg Expectation。 Halda-Jamuna，Jamuna-Brahmaputra孵化场，Jamuna-Raipur孵化场和Padma-Raipur孵化场之间存在显着的种群分化。根据遗传距离的UPGMA树状图显示了两个主要的集群：哈尔达河和赖布尔孵化场的人口在一个集群中，其余四个人口在另一个集群中。结论：这项研究总体上显示遗传水平较低孟加拉国三个主要河流和三个精选的C. cirrhosus孵化场种群的等位基因丰富度和杂合度方面的差异。

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