• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>British Biotechnology Journal >Non-Structured Kinetic Model ofAspergillus niger Growth and Substrate Uptakein a Batch Submerged Culture
【24h】

Non-Structured Kinetic Model ofAspergillus niger Growth and Substrate Uptakein a Batch Submerged Culture

机译：分批淹没培养中黑曲霉生长和底物吸收的非结构动力学模型

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aims: To investigate cell growth profile of Aspergillus niger in a batch submerged culture medium and then evaluation of cell kinetic behavior using some different non-structured kinetic models. Methodology: Experiments of cell growth and substrate utilization were conducted in batch submerged cultures with identified medium composition. Fitness assessment of experimental data on the cell growth and glucose consumption by models was performed using the curve-fitting tool in Mat Lab software. This report is the first in the kinetic investigation of Aspergillus niger PTCC 5010 with the studied models. This work was performed in Department of Chemical Engineering, Islamic Azad University, Qaemshahr Branch between April 2013 to September 2013. Results: Based on the obtained results; Moser kinetic model with R2 equal to 0.913 and Gompertz kinetic model with R2 equal to 0.949 were the best fitted models to describe the growth behavior of Aspergillus niger PTCC 5010 in the applied culture condition. Maximum specific cell growth rate with Moser and Gompertz kinetic models were 0.024 and 0.003h-1, respectively. Other kinetic constants for all studied models were also determined at the applied culture conditions. The consistency of the experimental data with Monod, Verhulst and Contois kinetic models wasn't in an acceptable range. Conclusion: In scale up biochemical projects by Aspergillus niger PTCC 5010 for some industrial products, Moser and Gompertz kinetic models are able to demonstrate cell growth behavior and its substrate uptake profile. In continuous processes, dilution rate could be determined based on obtained maximum specific cell growth rate equal to 0.024 h-1.
机译：目的：研究黑曲霉在分批浸没培养基中的细胞生长概况，然后使用一些不同的非结构动力学模型评估细胞动力学行为。方法：在具有确定培养基成分的分批浸没式培养物中进行细胞生长和底物利用的实验。使用Mat Lab软件中的曲线拟合工具，通过模型对细胞生长和葡萄糖消耗的实验数据进行适合性评估。该报告是使用研究模型对黑曲霉PTCC 5010进行动力学研究的第一份报告。这项工作是在2013年4月至2013年9月之间在伊斯兰阿扎德大学化学工程系Qaemshahr分校进行的。 R2等于0.913的Moser动力学模型和R2等于0.949的Gompertz动力学模型是描述黑曲霉PTCC 5010在应用培养条件下生长行为的最佳拟合模型。 Moser和Gompertz动力学模型的最大比细胞生长速率分别为0.024和0.003h-1。在所应用的培养条件下，还确定了所有研究模型的其他动力学常数。实验数据与Monod，Verhulst和Contois动力学模型的一致性不在可接受的范围内。结论：在黑曲霉PTCC 5010用于某些工业产品的大规模生化项目中，Moser和Gompertz动力学模型能够证明细胞生长行为及其底物摄取特征。在连续过程中，可以根据获得的最大比细胞生长速率等于0.024 h-1来确定稀释率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号