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首页> 外文期刊>Journal of industrial microbiology & biotechnology >Modeling growth, substrate consumption and product formation of Penicillium nalgiovense grown on meat simulation medium in submerged batch culture.
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Modeling growth, substrate consumption and product formation of Penicillium nalgiovense grown on meat simulation medium in submerged batch culture.

机译：模拟在淹没式分批培养中在肉类模拟培养基上生长的纳尔青霉的生长，底物消耗和产品形成。

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Penicillium nalgiovense is the most widely used starter mold for cured and fermented meat products. The development of a biomass film on the surface of these products prevents a large degree undesirable growth of various fungal contaminants and contributes to the ripening process with production of metabolites. This work presents an attempt to model the growth of P. nalgiovense and to relate it to substrate consumption and product release. Because of the extremely complex nature of the meat product fermentation, submerged culture was employed in a bioreactor system that enabled on-line monitoring, using a meat simulation medium, which contained peptones and lactate as carbon, nitrogen and energy sources. The unstructured model presented is based on a partial association of substrate assimilation and product formation with growth. Experimentally derived values for peptones and lactate were compared with model-derived values and their proportions corresponding to growth associated parts, used for biosynthesis, and non-growth associated parts, used for maintenance. The model was applied for the products ammonia, carbon dioxide and protons. Both peptones and lactate were used mainly for biosynthesis (85 and 80% of the total amounts provided, respectively). Assimilation of lactate and ammonia formation from amino acid metabolism resulted in a proton exchange, which was mainly growth associated. The contribution of the growth associated mechanism to the total proton exchange was estimated to be 75% while the contribution of the non-growth associated mechanism increased during the growth phase and reached a maximum of 25%. For carbon dioxide production, the contribution of a maintenance mechanism was evident at 40 h, while production was growth-associated and remained such even at the end of fermentation at 168 h when growth rate was very low. The partially growth associated model showed good agreement with the experimental data and allows accurate determination of the proportions of substrates or products related to biosynthesis and cell maintenance..
机译：纳尔尼奥青霉菌是用于腌制和发酵肉制品的最广泛使用的起子模具。在这些产品的表面上形成生物质膜可以防止各种真菌污染物的大量不良生长，并有助于代谢产物的成熟过程。这项工作提出了对纳尔体育假单胞菌生长进行建模并将其与底物消耗和产品释放联系起来的尝试。由于肉类产品发酵的性质极其复杂，因此在生物反应器系统中采用了浸入式培养，该系统可以使用包含蛋白medium和乳酸作为碳，氮和能源的肉类模拟介质进行在线监测。提出的非结构化模型基于底物同化和产物形成与生长的部分关联。将蛋白and和乳酸的实验得出的值与模型得出的值进行比较，并将它们的比例与用于生物合成的生长相关部分和用于维护的非生长相关部分相对应。该模型应用于产品氨，二氧化碳和质子。蛋白p和乳酸都主要用于生物合成（分别占提供总量的85％和80％）。氨基酸代谢产生的乳酸和氨同化导致质子交换，这主要与生长相关。生长相关机制对总质子交换的贡献估计为75％，而非生长相关机制的贡献在生长阶段增加，最高达到25％。对于二氧化碳的生产，维持机制的作用在40 h时就很明显，而生产是与生长相关的，甚至在生长速率很低的168 h发酵结束时也是如此。与部分生长相关的模型显示出与实验数据的良好一致性，并允许准确确定与生物合成和细胞维持有关的底物或产物的比例。

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