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首页> 外文期刊>British Biotechnology Journal >Molecular Identification of Biodiversity ofFusarium species Isolated fromWilted Oil Palm and Date Palm in Nigeria
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Molecular Identification of Biodiversity ofFusarium species Isolated fromWilted Oil Palm and Date Palm in Nigeria

机译：尼日利亚油棕和枣椰中us属植物生物多样性的分子鉴定

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Biodiversity of sixteen Fusarium isolates, isolated from the roots of oil palm and date palm in Nigeria was studied. Ten Fusarium isolates out of the sixteen were obtained from the oil palm while six isolates were from date palm. Random Amplified Polymorphic DNA (RAPD) was used to detect the phylogenetic similarity between them. The unweighted Pair Group Method with Arithmetic average (UPGMA) dendrogram clearly separated these sixteen Fusarium isolates into five groups (clusters). The first at SC values of 100 grouped six Fusarium isolates (5, 2, 13, 9, 8 and 14,) of both oil and date palms. They belonged to F. oxysporum and F. chlamydosporum. The second at SC values of 100 had one Fusarium isolate (16) of date palm. It belonged to F. solani. The third at DC values of 71-100 grouped six isolates (6, 4, 3, 7, 11 and 10) of oil and date palms. They all belonged to F. solani. The fourth at DC value of 76 had one Fusarium isolate (1) of oil palm, it belonged to F. nelsonii. The fifth at SC value of 100 grouped two Fusarium isolates (15,12) of date palm. They belonged to F. nelsonii and chlamydosporum. The sequence alignment technique showed there were similarities between these sixteen Fusarium isolates. The analysis of RAPD showed that the sixteen Fusarium isolates were five groups while sequence alignment technique grouped them into five Fusarium isolates. The slight difference in similarity may be attributed to ecological origin and zone of the Fusarium isolates.
机译：研究了从尼日利亚油棕和枣棕的根中分离出来的16种镰刀菌的生物多样性。从油棕中获得了16种中的10种镰刀菌分离株，而从椰棕中获得了6种分离株。随机扩增多态性DNA（RAPD）用于检测它们之间的系统发育相似性。用算术平均（UPGMA）树状图进行的非加权成对分组法将这16个镰刀菌分离株清楚地分为五个组（簇）。第一个在SC值上的油棕和枣椰子的100个分组的六个镰刀菌分离株（5、2、13、9、8和14）组合在一起。它们属于F.oxysporum和F.chlamydosporum。 SC值为100的第二个具有一个枣椰子镰刀菌分离株（16）。它属于F. solani。 DC值为71-100的第三组将油棕和枣椰子的六个分离株（6、4、3、7、11和10）分组。他们都属于F. solani。 DC值为76的第四个具有一个油棕镰孢镰刀菌分离株（1），它属于纳氏镰刀菌。在SC值为100的第五个中，将两个椰枣镰刀菌分离株（15,12）分组。它们属于纳氏菌和衣原体。序列比对技术显示这十六种镰刀菌分离株之间存在相似性。 RAPD分析表明，该16株枯萎病菌为5个类群，而序列比对技术将其分为5个枯萎病菌种。相似性的细微差异可能归因于镰刀菌分离物的生态起源和区域。

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