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首页> 外文期刊>Molecular Breeding >Identification of the molecular genetic basis of the low palmitic acid seed oil trait in soybean mutant line RG3 and association analysis of molecular markers with elevated seed stearic acid and reduced seed palmitic acid.
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Identification of the molecular genetic basis of the low palmitic acid seed oil trait in soybean mutant line RG3 and association analysis of molecular markers with elevated seed stearic acid and reduced seed palmitic acid.

机译：鉴定大豆突变株RG3中低棕榈酸种子油性状的分子遗传基础，并与增加的种子硬脂酸和减少的种子棕榈酸的分子标记进行关联分析。

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摘要

The fatty acid composition of vegetable oil is becoming increasingly critical for its ultimate functionality and utilization in foods and industrial products. Partial chemical hydrogenation of soybean [ Glycine max (L.) Merr.] oil increases oxidative stability and shelf life but also results in the introduction of trans fats as an unavoidable byproduct. Due to mandatory labeling of consumer products containing trans fats, conventional soybean oil has lost the ability to deliver the most appropriate economical functionality and oxidative stability, particularly for baking applications. Genetic improvement of the fatty acid profile of soybean oil is one method of meeting these new requirements for oil feedstocks. In this report, we characterized three mutant genetic loci controlling the saturated fatty acid content of soybean oil: two genes additively reduce palmitic acid content ( fap1 and fap3-ug), and one gene independently elevates stearic acid content ( fas). We identified a new null allele of fap3-ug/ GmFATB1A (derived from line ELLP2) present in line RG3. The splicing defect mutation in a beta-ketoacyl-[ acyl-carrier-protein] synthase III candidate gene located in the region mapped to fap1, derived originally from ethyl methane sulphonate mutant line C1726 (Cardinal et al. in Theor Appl Genet 127:97-111, 2014), was also present in line RG3. We also utilized the elevated stearic acid line RG7, which has previously been shown to contain novel mutant fas/ SACPD-C alleles encoding stearoyl-acyl carrier protein desaturase (Boersma et al. in Crop Sci 52:1736-1742, 2012). Molecular marker assays have been developed to track these causative mutations and understand their contributions to seed oil fatty acid profiles in a recombinant inbred line population segregating for fap1, fap3-ug, and fas alleles.
机译：植物油的脂肪酸组成对其最终功能和在食品和工业产品中的利用正变得越来越关键。大豆[Glycine max（L.）Merr。]油的部分化学加氢增加了氧化稳定性和保质期，但也导致了反式脂肪作为不可避免的副产物的引入。由于必须对含有反式脂肪的消费品进行强制性标签，常规大豆油已经失去了提供最合适的经济功能和氧化稳定性的能力，尤其是在烘烤应用中。大豆油脂肪酸谱的遗传改良是满足这些油原料新要求的方法之一。在本报告中，我们描述了控制大豆油中饱和脂肪酸含量的三个突变遗传基因座：两个基因可累加降低棕榈酸含量（fap1和fap3-ug），一个基因独立地提高硬脂酸含量（fas）。我们鉴定了RG3系中存在的fap3-ug / GmFATB1A新无效等位基因（来自ELLP2系）。位于映射到fap1的区域中的β-酮酰基-[酰基载体蛋白]合酶III候选基因中的剪接缺陷突变，最初源自甲烷磺酸乙酯突变株C1726（Cardinal等人，Theor Appl Genet 127：97 -111，2014年）也出现在RG3行中。我们还利用了升高的硬脂酸系RG7，该硬脂酸系RG7先前已显示出含有编码硬脂酰基-酰基载体蛋白去饱和酶的新型突变fas / SACPD-C等位基因（Boersma et al。in Crop Sci 52：1736-1742，2012）。已经开发了分子标记测定法，以追踪这些致病突变，并了解它们对分离为fap1，fap3-ug和fas等位基因的重组自交系种群中种子油脂肪酸谱的贡献。

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