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首页> 外文期刊>British Biotechnology Journal >Fungal Infection Enhances AnthocyaninProduction in Blue Flowers of Clitoria ternatea
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Fungal Infection Enhances AnthocyaninProduction in Blue Flowers of Clitoria ternatea

机译：真菌感染增强了Clitoria ternatea蓝花中花色苷的产生

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Aims: A white fungus which commonly infects blue petals of Clitoria ternatea L. (CT) was identified and total anthocyanin content and antioxidant activity of CT extracts from infected and uninfected CT were compared. Study Design: Experimental research.Place and Duration of Study: Samples of infected and non-infected CT were collected from Muang, Khon Kaen and chemical analysis was undertaken at Khon Kaen University between July and December 2012. Fungus was identified at Department of Microbiology in April 2013.Methodology: Samples of dried CT, with and without white fungus infection, were collected. The CT-infected fungus was isolated for microscopic characterization and DNA isolated for amplifying of its ITS rDNA, and analyzing their sequences with neighbor joining phylogenetic tree. Total anthocyanin content by pH differential method and antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay were used to compare infected and non-infected CT extracts in comparison to trolox. Both extracts were analyzed by HPLC in comparison to cyanidin.Results: Morphology and DNA of the infected fungus was characterized and confirmed to be Penicillium citrinum KKU-Rx1. In comparison to aqueous extracts of non-infected CT, infected CT gave about 1.5 times higher total anthocyanin content (P=.002) and cyanidin (P = .009). Trolox equivalent antioxidant capacity of infected-CT extracts was 1, while that of non-infected CT extracts was 0.7 (P<.001). Conclusion: Penicillium citrinum was identified from white fungus infection on deep blue flowers of Clitoria ternatea and shown to enhance the anthocyanin content and antioxidant activity of the CT extracts.
机译：目的：鉴定出一种常见的真菌，该真菌感染了Clitoria ternatea L.（CT）的蓝色花瓣，并比较了感染和未感染的CT提取物的总花色苷含量和抗氧化活性。研究设计：实验研究。研究的地点和时间：从Muang，Khon Kaen收集感染和未感染的CT样本，并于2012年7月至2012年12月在Khon Kaen大学进行了化学分析。真菌在2008年12月的微生物学系进行鉴定。 2013年4月方法：收集有无真菌感染的干CT样品。分离出CT感染的真菌，以进行显微镜鉴定，并分离出DNA，以扩增其ITS rDNA，并用邻居加入的系统树分析其序列。通过pH差示法测得的总花色苷含量和2,2-二苯基-1-吡啶并肼基（DPPH）测定的抗氧化活性用于比较受感染和未受感染的CT提取物与trolox。结果：鉴定了被感染真菌的形态和DNA，鉴定为柠檬青霉KKU-Rx1。与未感染CT的水提物相比，被感染CT的总花色苷含量（P = .002）和花青素（P = .009）约高1.5倍。被感染的CT提取物的Trolox当量抗氧化能力为1，而未被感染的CT提取物的Trolox当量抗氧化能力为0.7（P <.001）。结论：柠檬青霉是从深部Clitoria ternatea的深蓝色花朵上的白木耳感染中鉴定出的，并且可以增强CT提取物的花青素含量和抗氧化活性。

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