• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>British Biotechnology Journal >Non-target Host Immune Gene Modulation in Transgenic Silkworm Bombyx mori Endowed with RNAi Silence BmNPV Genes
【24h】

Non-target Host Immune Gene Modulation in Transgenic Silkworm Bombyx mori Endowed with RNAi Silence BmNPV Genes

机译：赋予RNAi沉默BmNPV基因的转基因家蚕中的非目标宿主免疫基因调节。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aim: To reveal differential expression of host- response genes activated after nuclear polyhedro virus infection in transgenic silkworm Bombyx mori larva and to show an influence of ‘ Bm NPV transgenes’ on expression pattern of host- response genes. Study Design: Relative expression profile of immune genes was analysed after Bm NPV infection in transgenic and non-transgenic larvae by real-time PCR. Place and Duration of Study: Genomics Division, Seribiotech Research Laboratory, Bangalore, India; 2014 January – 2016 December. Methodology: Expression of immune genes encoding components of Toll and melanisation pathways was analysed in third instar larvae of transgenic B. mori line mff118B by quantitative PCR at 0, 6, 9 and 24 h after Bm NPV infection and compared with infected non-transgenic larvae. A significant difference in relative expression was analyzed by Students' t – test or ANOVA and correlation in expression pattern, by linear regression in the probability distribution of Y as a function of X, at significance level P < 0.05. Results: In transgenic larvae, survival rate after NPV infection was up to 70% compared to 30% in non-transgenic larvae. Immune genes encoding NF-kappa B inhibition factor, Cactus, NF kappa B transcription factors, Dorsal and Relish, Toll- activating cytokine Sp?tzle, melanization pathway components, prophenol oxidase activating enzyme and prophenol oxidase1 showed significantly lower expression in Bm NPV infected- transgenic larva whereas enhanced expression in infected non-transgenic larvae. In infected transgenic larvae, selected gene pairs Cactus – Dorsal, Cactus – Relish, Spatzle - Dorsal, Spatzle - Cactus, Relish – Dorsal showed positively correlated expression whereas the correlation derailed in infected non-transgenic larvae. Conclusion: RNA interference-mediated inhibition of Bm NPV multiplication was engineered previously in B. mori . In infected transgenic silkworm, NPV multiplication rate is low and host-response genes showed low expression level. Under the influence of transgenes, host response genes showed correlated expression thus transgenes preserve specific host- gene interactions after NPV infection. Notably influence of ‘ Bm NPV transgenes' on expression of host response gene is a crucial revelation in the field of transgenesis to develop better antiviral resistance in silkworms.
机译：目的：揭示核多角体病毒感染后激活的宿主反应基因在家蚕家蚕幼虫中的差异表达，并显示“ Bm NPV转基因”对宿主反应基因表达模式的影响。研究设计：通过实时PCR分析Bm NPV感染转基因和非转基因幼虫后免疫基因的相对表达谱。研究地点和时间：印度班加罗尔Seribiotech研究实验室基因组学部; 2014年1月– 2016年12月。方法：在Bm NPV感染后0、6、9和24 h，通过定量PCR分析了转基因桑蚕品系mff118B的三龄幼虫中编码Toll和黑化途径成分的免疫基因的表达，并与感染的非转基因幼虫进行了比较。通过Student's t检验或ANOVA分析相对表达的显着差异，并通过显着性水平P <0.05的Y概率分布随X的线性回归分析表达模式的相关性。结果：在转基因幼虫中，NPV感染后的存活率高达70％，而非转基因幼虫为30％。编码NF-κB抑制因子，仙人掌，NF-κB转录因子，背侧和口齿，Toll激活细胞因子Spttzle，黑色素化途径成分，前酚氧化酶激活酶和前酚氧化酶1的免疫基因在Bm NPV感染的患者中表达明显降低。转基因幼虫，而在受感染的非转基因幼虫中表达增强。在感染的转基因幼虫中，选定的基因对仙人掌–背，仙人掌–丽珠，Spatzle –背，Spatzle –仙人掌，丽珠–背显示了正相关的表达，而相关在被感染的非转基因幼虫中脱轨。结论：RNA干扰介导的对Bm NPV增殖的抑制是先前在桑蚕中进行的。在感染的转基因家蚕中，NPV的繁殖率很低，宿主反应基因的表达水平也很低。在转基因的影响下，宿主反应基因显示出相关的表达，因此在NPV感染后，转基因保留了特定的宿主基因相互作用。 “ Bm NPV转基因”对宿主反应基因表达的显着影响是转基因领域发展出更好的家蚕抗病毒抗性的重要启示。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号