• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>British Biotechnology Journal >Effect of Low Frequency ElectromagneticFields (LF-EMFs) on c-myc OncogeneExpression Level in Peripheral BloodMononuclear Cells (PBMCs)
【24h】

Effect of Low Frequency ElectromagneticFields (LF-EMFs) on c-myc OncogeneExpression Level in Peripheral BloodMononuclear Cells (PBMCs)

机译：低频电磁场（LF-EMFs）对外周血单核细胞（PBMCs）c-myc癌基因表达水平的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Low frequency electromagnetic fields (LF-EMFs) which can be generated from homes and workplaces appliances can lead to alteration in oncogenes causing cancer diseases such as leukemia, nervous system tumors, lymphoma and breast cancer. To investigate the effect of LF-EMFs on c-myc oncogene expression level, primary cell culture of human peripheral blood mononuclear cells (PBMCs) were exposed to AC (50 Hz) electromagnetic flux density: 0.37mT, 0.82mT, 1.22mT, 1.68mT, 2.1mT, 2.47mT, 2.85mT, 3.33mT, 3.72mT, 3.92mT, 4.32mT and 4.67mT using exposure unit. After four days of exposure, when initial changes in cell viability, morphology and count between exposed and unexposed cells were noted, total RNA was extracted to evaluate the expression level of c-myc oncogene by quantitative real time PCR (qRT-PCR). The results showed that c-myc oncogene expression level began to increase gradually from value 1.69% at 0.82mT reaching to the maximum expression level value 4.65% at 4.67mT. This result refers to an increasing in LF-EMFs exposure offset by an increasing in c-myc oncogene expression level, affecting different cellular functions. This study indicates that LF-EMF is environmental pollution effects on the expression level of oncogenes which increase the risk of human cancer diseases.
机译：可能从家庭和工作场所的设备中产生的低频电磁场（LF-EMF）会导致致癌基因发生变化，从而导致癌症疾病，例如白血病，神经系统肿瘤，淋巴瘤和乳腺癌。为了研究LF-EMF对c-myc癌基因表达水平的影响，将人类外周血单核细胞（PBMC）的原代细胞培养物暴露于AC（50 Hz）电磁通量密度下：0.37mT，0.82mT，1.22mT，1.68 mT，2.1mT，2.47mT，2.85mT，3.33mT，3.72mT，3.92mT，4.32mT和4.67mT。暴露四天后，当注意到细胞活力，形态和暴露与未暴露细胞之间的计数的初始变化时，通过定量实时PCR（qRT-PCR）提取总RNA以评估c-myc癌基因的表达水平。结果表明，c-myc癌基因表达水平开始逐渐增加，从0.82mT时的1.69％上升到4.67mT时的最大表达水平4.65％。该结果是指通过增加c-myc癌基因表达水平，影响不同的细胞功能，LF-EMFs暴露抵消增加。这项研究表明，LF-EMF是环境污染对癌基因表达水平的影响，这增加了人类癌症疾病的风险。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号