• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>British Biotechnology Journal >In vitro Antitheilerial Activity of Paluther(Artemether 80) Against Theileria lestoquardi
【24h】

In vitro Antitheilerial Activity of Paluther(Artemether 80) Against Theileria lestoquardi

机译：Paluther（蒿甲醚80）的体外抗莱斯泰勒泰勒氏菌的抗菌活性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aim: The aim of this study was to screen Artemether 80 for activity against Theileria lestoquardi (Apicomplexa: Theileridae) using buparvaquone as a standard drug.Study Design: In vitro study under laboratories conditions.Place and Duration of Study: Veterinary Research Institute, between 2006 and 2008. Methodology: Artemether 80 was screened for the first time to investigate activity against T. lestoquardi at different concentrations. Blood was collected separately from normal sheep and sheep infected naturally with Theileria. Normal lymphocyte cells and lymphocyte cells infected with Theileria were isolated from heparinized blood with Ficoll-paque. Isolated cells were grown in Minimum Essential Medium (MEM), supplemented with 20% calf serum and sub cultured. The parasite was identified with indirect fluorescent antibody test (IFA). A volume of 2.7 ml of lymphoblast cell suspension at concentration of 5x104 cell/ ml was distributed in tissue culture plates, and then 0.3 ml of drug at concentrations of 0.1, 1.0, 10 and 100 mg/L was added separately. A volume of 0.3 ml MEM was added to infected untreated control. Results: The in vitro antitheilerial activity of Artemether 80 against T. lestoquardi 48 h after exposure was 0%, 14%, 30% and 45% at concentrations of 0.01, 0.1, 1.0 and 10 mg/L, respectively as compared with activity of buparvaquone at the same concentrations being 74%, 83%, 92% and 100%, respectively. Both Artemether 80 and buparvaquone caused in vitro partial cytotoxic effect at the highest concentrations. Activity and/ or partial cytotoxic effect of both drugs caused changes in the morphology of macroscizonts and host lymphoblast cells, decreased the number of macroschizonts/cell, mean number of dividing cells, increased the number of cells with extra cellular macroschizonts.Conclusion: It was concluded that Artemether 80 is slightly effective in vitro against T. lestoquardi.
机译：目的：本研究的目的是使用丁草胺作为标准药物筛选Artemether 80对莱斯泰夸氏菌（Apicomplexa：Theileridae）的活性研究设计：在实验室条件下的体外研究研究地点和持续时间：兽医研究所2006年和2008年。方法：首次筛选了Artemether 80，以研究不同浓度下对T. lestoquardi的活性。从正常绵羊和自然感染了泰勒虫的绵羊中分别采集血液。用Ficoll-paque从肝素化血液中分离出正常的淋巴细胞和被泰勒虫感染的淋巴细胞。分离的细胞在补充有20％小牛血清的最低必需培养基（MEM）中生长，并进行亚培养。通过间接荧光抗体测试（IFA）鉴定了该寄生虫。将体积为5x104细胞/ ml的2.7 ml淋巴母细胞细胞悬液分配到组织培养板中，然后分别添加浓度为0.1、1.0、10和100 mg / L的0.3 ml药物。将0.3 ml MEM的体积添加到感染的未处理对照中。结果：浓度为0.01、0.1、1.0和10 mg / L时，蒿甲醚80暴露48h后对莱斯托夸迪的体外抗菌活性分别为0％，14％，30％和45％。在相同浓度下，丁草胺的活性分别为74％，83％，92％和100％。在最高浓度下，蒿甲醚80和丁丙诺啡都引起体外部分细胞毒性作用。两种药物的活性和/或部分细胞毒作用均导致大裂殖细胞和宿主淋巴母细胞的形态发生变化，大裂殖细胞/细胞数量减少，平均分裂细胞数量，具有额外细胞大裂殖细胞的细胞数量增加。结论是Artemether 80在体外对T. lestoquardi有轻微的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号