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首页> 外文期刊>BMC Microbiology >Extraintestinal pathogenic Escherichia coli O1:K1:H7/NM from human and avian origin: detection of clonal groups B2 ST95 and D ST59 with different host distribution
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Extraintestinal pathogenic Escherichia coli O1:K1:H7/NM from human and avian origin: detection of clonal groups B2 ST95 and D ST59 with different host distribution

机译：来自人和鸟类的肠外致病性大肠杆菌O1：K1：H7 / NM：检测具有不同宿主分布的克隆组B2 ST95和D ST59

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Background Extraintestinal pathogenic Escherichia coli (ExPEC) strains of serotype O1:K1:H7/NM are frequently implicated in neonatal meningitis, urinary tract infections and septicemia in humans. They are also commonly isolated from colibacillosis in poultry. Studies to determine the similarities of ExPEC from different origins have indicated that avian strains potentially have zoonotic properties. Results A total of 59 ExPEC O1:K1:H7/NM isolates (21 from avian colibacillosis, 15 from human meningitis, and 23 from human urinary tract infection and septicemia) originated from four countries were characterized by phylogenetic PCR grouping, Multilocus Sequence Typing (MLST), Pulsed Field Gel Electrophoresis (PFGE) and genotyping based on several genes known for their association with ExPEC or avian pathogenic Escherichia coli (APEC) virulence. APEC and human ExPEC isolates differed significantly in their assignments to phylogenetic groups, being phylogroup B2 more prevalent among APEC than among human ExPEC (95% vs. 53%, P = 0.001), whereas phylogroup D was almost exclusively associated with human ExPEC (47% vs. 5%, P = 0.0000). Seven virulence genes showed significant differences, being fimAvMT78 and sat genes linked to human isolates, while papGII, tsh, iron, cvaC and iss were significantly associated to APEC. By MLST, 39 of 40 ExPEC belonging to phylogroup B2, and 17 of 19 belonging to phylogroup D exhibited the Sequence Types (STs) ST95 and ST59, respectively. Additionally, two novel STs (ST1013 and ST1006) were established. Considering strains sharing the same ST, phylogenetic group, virulence genotype and PFGE cluster to belong to the same subclone, five subclones were detected; one of those grouped six strains of human and animal origin from two countries. Conclusion Present results reveal that the clonal group B2 O1:K1:H7/NM ST95, detected in strains of animal and human origin, recovered from different dates and geographic sources, provides evidence that some APEC isolates may act as potential pathogens for humans and, consequently, poultry as a foodborne source, suggesting no host specificity for this type of isolates. A novel and important finding has been the detection of the clonal group D O1:K1:H7/NM ST59 almost exclusively in humans, carrying pathogenic genes linked to the phylogenetic group D. This finding would suggest D O1:K1:H7/NM ST59 as a host specific pathotype for humans.
机译：背景血清型O1：K1：H7 / NM的肠外致病性大肠杆菌（ExPEC）菌株通常与人类新生儿脑膜炎，尿路感染和败血病有关。它们通常也从家禽大肠杆菌病中分离出来。确定不同来源的ExPEC相似性的研究表明，禽毒株可能具有人畜共患病特性。结果来自四个国家的59株ExPEC O1：K1：H7 / NM分离株（来自禽大肠杆菌病的21株，来自人脑膜炎的15株，来自人尿道感染和败血病的23株）通过系统进化PCR分组，多基因座序列分型（ MLST），脉冲场凝胶电泳（PFGE）和基因分型，这些基因基于与ExPEC或禽病原性大肠杆菌（APEC）毒力相关的几种已知基因。 APEC和人类ExPEC分离株在系统发育组中的分配有显着差异，在APEC中，进化组B2比人类ExPEC中更为普遍（95％比53％，P = 0.001），而进化组D几乎完全与人类ExPEC相关（47 ％对5％，P = 0.0000）。七个毒力基因显示出显着差异，其中fimAv MT78 和sat基因与人类分离株有关，而papGII，tsh，铁，cvaC和iss与APEC显着相关。通过MLST，属于phylogroup B2的40个ExPEC中的39个和属于phylogroup D的19个中的17个分别显示了序列类型（ST）ST95和ST59。另外，建立了两个新颖的ST（ST1013和ST1006）。考虑到具有相同ST，系统发育组，毒力基因型和PFGE簇的菌株属于同一亚克隆，共检测到5个亚克隆。其中一组来自两个国家的六种人类和动物来源的菌株。结论目前的结果表明，在动物和人类起源的菌株中检测到的克隆组B2 O1：K1：H7 / NM ST95可从不同的日期和地理来源回收，这提供了一些APEC分离株可能是人类潜在病原体的证据，并且因此，家禽是一种食源性来源，表明这种分离株没有宿主特异性。一个新的重要发现是检测到克隆群D O1：K1：H7 / NM ST59几乎只在人类中携带带有与系统发生群D相关的致病基因。该发现表明D O1：K1：H7 / NM ST59作为人类的宿主特异性病原体。

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