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首页> 外文期刊>BMC Microbiology >Induction of a chemoattractant transcriptional response by a Campylobacter jejuni boiled cell extract in colonocytes
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Induction of a chemoattractant transcriptional response by a Campylobacter jejuni boiled cell extract in colonocytes

机译：空肠弯曲杆菌煮沸的细胞提取物在结肠细胞中诱导趋化转录反应

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Background Campylobacter jejuni, the commonest cause of bacterial diarrhoea worldwide, can also induce colonic inflammation. To understand how a previously identified heat stable component contributes to pro-inflammatory responses we used microarray and real-time quantitative PCR to investigate the transcriptional response to a boiled cell extract of Campylobacter jejuni NCTC 11168. Results RNA was extracted from the human colonocyte line HCA-7 (clone 29) after incubation for 6 hours with Campylobacter jejuni boiled cell extract and was used to probe the Affymetrix Human Genome U133A array. Genes differentially affected by Campylobacter jejuni boiled cell extract were identified using the Significance Score algorithm of the Bioconductor software suite and further analyzed using the Ingenuity Pathway Analysis program. The chemokines CCL20, CXCL3, CXCL2, Interleukin 8, CXCL1 and CXCL6 comprised 6 of the 10 most highly up-regulated genes, all with Significance Scores ≥ 10. Members of the Tumor Necrosis Factor α/Nuclear Factor-κB super-family were also significantly up-regulated and involved in the most significantly regulated signalling pathways (Death receptor, Interleukin 6, Interleukin 10, Toll like receptor, Peroxisome Proliferator Activated Receptor-γ and apoptosis). Ingenuity Pathway Analysis also identified the most affected functional gene networks such as cell movement, gene expression and cell death. In contrast, down-regulated genes were predominantly concerned with structural and metabolic functions. Conclusion A boiled cell extract of Campylobacter jejuni has components that can directly switch the phenotype of colonic epithelial cells from one of resting metabolism to a pro-inflammatory one, particularly characterized by increased expression of genes for leukocyte chemoattractant molecules.
机译：背景空肠弯曲杆菌是全世界细菌性腹泻的最常见原因，也可诱发结肠炎症。为了解先前鉴定的热稳定成分如何促炎反应，我们使用微阵列和实时定量PCR研究了空肠弯曲杆菌NCTC 11168对煮沸细胞提取物的转录反应。结果从人结肠细胞株HCA中提取RNA与空肠弯曲杆菌煮沸的细胞提取液一起孵育6小时后，将-7（克隆29）用于Affymetrix Human Genome U133A阵列。空肠弯曲杆菌煮沸的细胞提取物差异影响的基因使用Bioconductor软件套件的“重要性评分”算法进行鉴定，并使用“机能途径分析”程序进行进一步分析。趋化因子CCL20，CXCL3，CXCL2，白介素8，CXCL1和CXCL6包含10个上调程度最高的基因中的6个，其显着性得分≥10。肿瘤坏死因子α/核因子κB超家族成员也显着上调并参与最显着调节的信号传导途径（死亡受体，白介素6，白介素10，Toll样受体，过氧化物酶体增殖物激活的受体-γ和凋亡）。创造力途径分析还确定了受影响最大的功能基因网络，例如细胞运动，基因表达和细胞死亡。相反，下调的基因主要与结构和代谢功能有关。结论空肠弯曲菌煮沸的细胞提取物具有可直接将结肠上皮细胞表型从静止代谢的一种转变为促炎性的成分，其特征在于白细胞趋化分子的基因表达增加。

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