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首页> 外文期刊>BMC Microbiology >Role of subtyping in detecting Salmonella cross contamination in the laboratory
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Role of subtyping in detecting Salmonella cross contamination in the laboratory

机译：亚型在实验室检测沙门氏菌交叉污染中的作用

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Background With the exception of M. tuberculosis, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National Salmonella Reference Laboratory (Ireland) from 2000–2007 to identify likely incidents of laboratory cross contamination. Methods Serotyping and antimicrobial susceptibility testing was performed on all Salmonella isolates received in the NSRL. Phage typing was performed on all S. Typhimurium and S. Enteritidis isolates while multi-locus variance analysis (MLVA) was performed on selected S. Typhimurium isolates. Pulsed field gel electrophoresis (PFGE) using the PulseNet standard protocol was performed on selected isolates of various serovars. Results Twenty-three incidents involving fifty-six isolates were identified as likely to represent cross contamination. The probable sources of contamination identified were the laboratory positive control isolate (n = 13), other test isolates (n = 9) or proficiency test samples (n = 1). Conclusion The scale of laboratory cross-contamination in bacteriology is most likely under recognized. Testing laboratories should be aware of the potential for cross-contamination, regularly review protocols to minimize its occurrence and consider it as a possibility when unexpected results are obtained.
机译：背景技术除结核分枝杆菌外，在细菌学实验室中关于交叉污染问题的报道很少。我们对2000-2007年国家沙门氏菌参考实验室（爱尔兰）的亚型数据进行了回顾性分析，以确定可能发生实验室交叉污染的事件。方法对NSRL中所有沙门氏菌分离物进行血清分型和抗菌药敏试验。对所有鼠伤寒沙门氏菌和肠炎沙门氏菌分离株进行噬菌体分型，而对所选鼠伤寒沙门氏菌分离株进行多位点方差分析（MLVA）。使用PulseNet标准协议的脉冲场凝胶电泳（PFGE）在各种血清型的选定分离物中进行。结果经鉴定，有23例涉及56株分离株的事件很可能代表交叉污染。确定的可能污染源是实验室阳性对照分离物（n = 13），其他测试分离物（n = 9）或能力验证样品（n = 1）。结论细菌学实验室交叉污染的规模很可能尚未得到认可。测试实验室应意识到交叉污染的可能性，定期检查方案以最大程度地减少交叉污染的发生，并在获得意外结果时将其视为可能。

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