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首页> 外文期刊>BMC Microbiology >Quantitative proteomics of nutrient limitation in the hydrogenotrophic methanogen Methanococcus maripaludis
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Quantitative proteomics of nutrient limitation in the hydrogenotrophic methanogen Methanococcus maripaludis

机译：氢营养型产甲烷菌马氏甲烷球菌营养限制的定量蛋白质组学

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Background Methanogenic Archaea play key metabolic roles in anaerobic ecosystems, where they use H2 and other substrates to produce methane. Methanococcus maripaludis is a model for studies of the global response to nutrient limitations. Results We used high-coverage quantitative proteomics to determine the response of M. maripaludis to growth-limiting levels of H2, nitrogen, and phosphate. Six to ten percent of the proteome changed significantly with each nutrient limitation. H2 limitation increased the abundance of a wide variety of proteins involved in methanogenesis. However, one protein involved in methanogenesis decreased: a low-affinity [Fe] hydrogenase, which may dominate over a higher-affinity mechanism when H2 is abundant. Nitrogen limitation increased known nitrogen assimilation proteins. In addition, the increased abundance of molybdate transport proteins suggested they function for nitrogen fixation. An apparent regulon governed by the euryarchaeal nitrogen regulator NrpR is discussed. Phosphate limitation increased the abundance of three different sets of proteins, suggesting that all three function in phosphate transport. Conclusion The global proteomic response of M. maripaludis to each nutrient limitation suggests a wider response than previously appreciated. The results give new insight into the function of several proteins, as well as providing information that should contribute to the formulation of a regulatory network model.
机译：背景产甲烷古生菌在厌氧生态系统中发挥关键的代谢作用，在厌氧生态系统中，它们使用H 2 和其他底物来生产甲烷。 maripaloccus maripaludis是研究全球对营养限制的反应的模型。结果我们使用高覆盖率定量蛋白质组学来确定马氏念珠菌对H 2 ，氮和磷酸盐的生长限制水平的响应。每种营养素限制条件下，蛋白质组的百分之六到百分之十都会发生显着变化。 H 2 的局限性增加了参与甲烷生成的多种蛋白质的丰度。但是，参与甲烷生成的一种蛋白质减少了：低亲和力[Fe]氢化酶，当H 2 丰富时，它可能在高亲和力机制中占主导地位。氮限制增加了已知的氮同化蛋白。此外，钼酸盐转运蛋白丰度的增加表明它们可用于固氮。讨论了由延胡索氮调节剂NrpR控制的表观调节子。磷酸盐限制增加了三种不同蛋白质的丰度，表明这三种蛋白质均在磷酸盐转运中起作用。结论maripaludis对每种营养物质限制的总体蛋白质组学反应表明，其反应范围比以前认为的要广。结果为几种蛋白质的功能提供了新的见识，并提供了有助于监管网络模型制定的信息。

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