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首页> 外文期刊>BMC Plant Biology >Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean
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Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean

机译：互补的遗传和基因组方法有助于表征大豆中的I类连锁种子蛋白QTL

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Background The nutritional and economic value of many crops is effectively a function of seed protein and oil content. Insight into the genetic and molecular control mechanisms involved in the deposition of these constituents in the developing seed is needed to guide crop improvement. A quantitative trait locus (QTL) on Linkage Group I (LG I) of soybean ( Glycine max (L.) Merrill) has a striking effect on seed protein content. Results A soybean near-isogenic line (NIL) pair contrasting in seed protein and differing in an introgressed genomic segment containing the LG I protein QTL was used as a resource to demarcate the QTL region and to study variation in transcript abundance in developing seed. The LG I QTL region was delineated to less than 8.4 Mbp of genomic sequence on chromosome 20. Using Affymetrix? Soy GeneChip and high-throughput Illumina? whole transcriptome sequencing platforms, 13 genes displaying significant seed transcript accumulation differences between NILs were identified that mapped to the 8.4 Mbp LG I protein QTL region. Conclusions This study identifies gene candidates at the LG I protein QTL for potential involvement in the regulation of protein content in the soybean seed. The results demonstrate the power of complementary approaches to characterize contrasting NILs and provide genome-wide transcriptome insight towards understanding seed biology and the soybean genome.
机译：背景许多农作物的营养和经济价值实际上是种子蛋白质和油含量的函数。需要深入了解与这些成分在发育中的种子中沉积有关的遗传和分子控制机制，以指导作物改良。大豆（Glycine max（L.）Merrill）连锁I（LG I）的数量性状基因座（QTL）对种子蛋白质含量具有显着影响。结果使用大豆近等基因系（NIL）对，其种子蛋白质形成对比，而渗入的基因组片段中含有LG I蛋白质QTL，这两个基因被用来划定QTL区域并研究发育中种子转录本丰度的变化。 LG I QTL区划为20号染色体上少于8.4 Mbp的基因组序列。使用Affymetrix ？ Soy GeneChip和高通量Illumina ？全转录组测序平台，13 NIL之间显示出显着种子转录积累差异的基因被鉴定到8.4 Mbp LG I蛋白QTL区。结论本研究确定了LG I蛋白QTL的基因候选物，这些基因候选物可能参与了大豆种子中蛋白质含量的调节。结果表明，互补方法可表征不同的NIL，并为了解种子生物学和大豆基因组提供全基因组转录组见解。

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