首页> 外文期刊>Canadian Journal of Pure and Applied Sciences >ANALYSIS OF ANTIBIOTIC SUSCEPTIBILITY OF KLEBSIELLA PNEUMONIAE ISOLATED FROM DIFFERENT CLINICAL SPECIMEN IN ENUGU STATE
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ANALYSIS OF ANTIBIOTIC SUSCEPTIBILITY OF KLEBSIELLA PNEUMONIAE ISOLATED FROM DIFFERENT CLINICAL SPECIMEN IN ENUGU STATE

机译:恩乌古州不同临床标本分离的肺炎克雷伯菌肺炎克雷伯菌药敏性分析

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We analyzed the antibiotic susceptibility profile of one hundred and fifty (150) Klebsiella pneumoniae strains isolated from different clinical samples (urine= 72, high vaginal swab=12, sputum=50 and wound swab= 16) isolated from patients visiting University of Nigeria teaching hospital (UNTH) Enugu, during June 2008 – May 2009. All samples were analyzed and organism isolated using standard Microbiology techniques, antibiotic susceptibility testing was carried out as described in the manual of antibiotic susceptibility testing. Clonal relatedness of all the K. pneumoniae strains was determined by randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). Antibiotic susceptibility studies revealed that K. pneumoniae from wound samples were the most susceptible strains followed by HVS, sputum and urine. The overall susceptibility profile are as follows; imipenem (100%), amikacin (100%), cefoxitin (99.4%), aztreonam (98%), ceftazidime (98%), cefotaxime (96.7%), amoxicillin/clavulanic acid (96%), ciprofloxacin (96%), tobramycin (93.3%), kanamycin (90%), cefuroxime (86.7%), gentamicin (76%), sulphamethoxazole/trimethoprim (22%), chloramphenicol (15.4%) and ampicillin (5%). RAPD analysis to determine the clonal relatedness of resistance strains grouped them into two clusters (A and B) based on band patterns. All strains resistance to ampicillin and chloramphenicol showed 100% similarity in band patterns (clonal group A) while strains resistance to sulphamethoxazole/trimethoprim showed different band patterns (clonal group B). Our study revealed strains of Klebsiella pneumoniae belonging to two clonal groups based on their resistant to ampicillin, chloramphenicol and sulphamethoxazole/trimethroprim.
机译:我们分析了从访问尼日利亚大学教学的患者中分离出的不同临床样品(尿液= 72,高阴道拭子= 12,痰液= 50和伤口拭子= 16)中分离出的一百五十(150)株肺炎克雷伯菌的抗生素敏感性分布2008年6月至2009年5月,在Enugu的Enugu医院进行了分析。所有细菌均进行了分析,并使用标准微生物学技术分离了微生物,并按照《抗生素敏感性试验手册》中的说明进行了抗生素敏感性试验。通过随机扩增的多态性DNA聚合酶链反应(RAPD-PCR)确定所有肺炎克雷伯菌菌株的克隆相关性。抗生素敏感性研究表明,伤口样本中的肺炎克雷伯菌是最易感染的菌株,其次是HVS,痰液和尿液。总体敏感性概况如下:亚胺培南(100%),阿米卡星(100%),头孢西丁(99.4%),氨曲南(98%),头孢他啶(98%),头孢噻肟(96.7%),阿莫西林/克拉维酸(96%),环丙沙星(96%) ,妥布霉素(93.3%),卡那霉素(90%),头孢呋辛(86.7%),庆大霉素(76%),磺胺甲恶唑/甲氧苄啶(22%),氯霉素(15.4%)和氨苄西林(5%)。通过RAPD分析来确定抗性菌株的克隆相关性,基于条带模式将其分为两个簇(A和B)。所有菌株对氨苄青霉素和氯霉素的抗性均显示出100%的条带模式相似性(克隆组A),而菌株对磺胺甲恶唑/甲氧苄氨嘧啶的抗性均呈现出不同的条带类型(克隆性B组)。我们的研究显示,由于其对氨苄西林,氯霉素和磺胺甲恶唑/甲氧苄氨嘧啶的抗性,肺炎克雷伯氏菌菌株属于两个克隆群。

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