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首页> 外文期刊>Canadian Journal of Pure and Applied Sciences >MORPHOLOGICAL VARIATIONS IN COLONY AMONG CLINICAL CANDIDA STRAINS
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MORPHOLOGICAL VARIATIONS IN COLONY AMONG CLINICAL CANDIDA STRAINS

机译：临床念珠菌菌株克隆的形态学变化

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A study was undertaken to document the colony morphology (surface, shape, consistency, elevation and colour) in Candida strains. Samples were collected from symptomatic female patients in some cities in Nigeria. Microscopically- detected (direct mount wet preparation) Candida – positive samples (n=585) were used. The samples made up of high vaginal swabs (83%), endocervical swabs (14%) and urine (3%) were plated on Sabouraud Glucose Agar and incubated at 37 0 C for 48 hours. The surface and consistency types were determined by touching each colony with an inoculation loop (4mm). The cultures yielded 595 isolates of Candida, made up of seven species. Three colony surfaces were identified – Wet (W), Dry Shiny (DS) and Dry Powdery (DP) - with the DS having the highest distribution (65.1%) and DP, the least distribution (12.7%). Also, there were three distinguishable colony consistencies – Creamy (C), Dry Hard (DH), and Dry Grannular (DG) - with distribution of 87, 12 and 1%, respectively. Most of the species showed raised colonies. There were species - specific and intra- specific variations in surface and consistency. Statistically (p≤ 0.05), some of the colony features were species-dependent while others were not. The results provide an all-encompassing information on the colony morphological diversity in Candida species which is valuable in the understanding of the biology of Candida.
机译：进行了一项研究以记录念珠菌菌株的菌落形态（表面，形状，稠度，高度和颜色）。从尼日利亚一些城市的有症状女性患者中收集样本。用显微镜检测（直接湿法制备）假丝酵母–阳性样品（n = 585）。将由高阴道拭子（83％），宫颈内拭子（14％）和尿液（3％）组成的样品铺在Sabouraud葡萄糖琼脂上，并在37 0 C下孵育48小时。通过用接种环（4mm）接触每个菌落来确定表面和稠度类型。培养产生了595个念珠菌分离株，由7个种组成。确定了三个菌落表面-湿（W），干发亮（DS）和干粉状（DP）-DS的分布最高（65.1％），DP的分布最低（12.7％）。此外，存在三种可区分的菌落一致性-乳脂状（C），干硬质（DH）和干粒状（DG）-分布分别为87％，12％和1％。大多数物种显示出升高的菌落。在表面和稠度上存在物种（特定和内部特定）变化。统计学上（p≤0.05），某些菌落特征是物种依赖性的，而另一些则不是。结果提供了关于念珠菌物种的菌落形态多样性的全面信息，这对于了解念珠菌的生物学意义重大。

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