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首页> 外文期刊>Cellular Physiology and Biochemistry >Effect of Non-Esterified Fatty Acids on Fatty Acid Metabolism-Related Genes in Calf Hepatocytes Cultured in Vitro
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Effect of Non-Esterified Fatty Acids on Fatty Acid Metabolism-Related Genes in Calf Hepatocytes Cultured in Vitro

机译：非酯化脂肪酸对体外培养小牛肝细胞脂肪酸代谢相关基因的影响

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Background: NEFA plays numerous roles in the metabolism of glucose, lipids, and proteins. A number of experimental studies have shown that NEFA may have an important role in fatty acid metabolism in the liver, especially in dairy cows that experience negative energy balance (NEB) during early lactation. Methods: In this study, using fluorescent quantitative RT-PCR, ELISA, and primary hepatocytes cultured in vitro, we examined the effect of NEFA (0, 0.2, 0.4, 0.8, 1.6, and 3.2 mmol/L) on fatty acid metabolism by monitoring the mRNA and protein expression of the following key enzymes: long chain acyl-CoA synthetase (ACSL), carnitine palmitoyltransferase IA (CPT IA), long chain acyl-CoA dehydrogenase (ACADL), and acetyl-CoA carboxylase (ACC). Results: The mRNA and protein expression levels of ACSL and ACADL markedly increased as the concentration of NEFA in the media was increased. The mRNA and protein expression levels of CPT IA were enhanced significantly when the NEFA concentrations increased from 0 to 1.6 mmol/L and decreased significantly when the NEFA concentrations increased from 1.6 to 3.2 mmol/L. The mRNA and protein expression of ACC decreased gradually with increasing concentrations of NEFA. Conclusion: These findings indicate that increased NEFA significantly promote the activation and β-oxidation of fatty acids, but very high NEFA concentrations may inhibit the translocation of fatty acids into mitochondria of hepatocytes. This may explain the development of ketosis or liver lipidosis in dairy cows. CPT IA might be the key control enzyme of the fatty acid oxidation process in hepatocytes.
机译：背景：NEFA在葡萄糖，脂质和蛋白质的代谢中起着许多作用。许多实验研究表明，NEFA可能在肝脏的脂肪酸代谢中起重要作用，尤其是在哺乳初期经历负能量平衡（NEB）的奶牛。方法：在这项研究中，我们使用荧光定量RT-PCR，ELISA和体外培养的原代肝细胞，通过以下方法检测了NEFA（0、0.2、0.4、0.8、1.6和3.2 mmol / L）对脂肪酸代谢的影响。监测以下关键酶的mRNA和蛋白质表达：长链酰基辅酶A合成酶（ACSL），肉碱棕榈酰转移酶IA（CPT IA），长链酰基辅酶A脱氢酶（ACADL）和乙酰辅酶A羧化酶（ACC）。结果：随着培养基中NEFA浓度的增加，ACSL和ACADL的mRNA和蛋白质表达水平显着增加。当NEFA浓度从0增加到1.6 mmol / L时，CPT IA的mRNA和蛋白表达水平显着提高，而当NEFA浓度从1.6到3.2 mmol / L增加时CPT IA的mRNA和蛋白表达水平显着降低。随着NEFA浓度的增加，ACC的mRNA和蛋白表达逐渐降低。结论：这些发现表明增加的NEFA可以显着促进脂肪酸的活化和β-氧化，但是非常高的NEFA浓度可能会抑制脂肪酸向肝细胞线粒体的转运。这可以解释奶牛中的酮症或肝脂质增多的发展。 CPT IA可能是肝细胞脂肪酸氧化过程的关键控制酶。

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