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首页> 外文期刊>Cellular Physiology and Biochemistry >The Hypertonic Environment Differentially Regulates Wild-type CFTR and TNR-CFTR Chloride Channels
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The Hypertonic Environment Differentially Regulates Wild-type CFTR and TNR-CFTR Chloride Channels

机译：高渗环境差异性地调节野生型CFTR和TNR-CFTR氯化物通道

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This study tested the hypotheses that the hypertonic environment of the renal medulla regulates the expression of cystic fibrosis transmembrane conductance regulator protein (CFTR) and its natural splice variant, TNR-CFTR. To accomplish this, Madin-Darby canine kidney (MDCK) stable cell lines expressing TNR-CFTR or CFTR were used. The cells were treated with hypertonic medium made with either NaCl or urea or sucrose (480 mOsm/kg or 560 mOsm/kg) to mimic the tonicity of the renal medulla environment. Western blot data showed that CFTR and TNR-CFTR total cell protein is increased by hypertonic medium, but using the surface biotinylation technique, only CFTR was found to be increased in cell plasma membrane. Confocal microscopy showed TNR-CFTR localization primarily at the endoplasmic reticulum and plasma membrane. In conclusion, CFTR and TNR-CFTR have different patterns of distribution in MDCK cells and they are modulated by a hypertonic environment, suggesting their physiological importance in renal medulla.
机译：这项研究验证了肾髓质的高渗环境调节囊性纤维化跨膜电导调节蛋白（CFTR）及其天然剪接变体TNR-CFTR的表达的假设。为此，使用了表达TNR-CFTR或CFTR的Madin-Darby犬肾（MDCK）稳定细胞系。用由NaCl或尿素或蔗糖制成的高渗培养基（480 mOsm / kg或560 mOsm / kg）处理细胞，以模拟肾髓质环境的张力。蛋白质印迹数据表明，高渗介质可增加CFTR和TNR-CFTR的总细胞蛋白，但使用表面生物素化技术，仅发现CFTR在细胞质膜中增加。共聚焦显微镜显示TNR-CFTR主要位于内质网和质膜。总之，CFTR和TNR-CFTR在MDCK细胞中具有不同的分布模式，并且受到高渗环境的调节，表明它们在肾髓质中的生理重要性。

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