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首页> 外文期刊>Cellular Physiology and Biochemistry >The hypertonic environment differentially regulates wild-type CFTR and TNR-CFTR chloride channels
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The hypertonic environment differentially regulates wild-type CFTR and TNR-CFTR chloride channels

机译：高渗环境差异性调节野生型CFTR和TNR-CFTR氯化物通道

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This study tested the hypotheses that the hypertonic environment of the renal medulla regulates the expression of cystic fibrosis transmembrane conductance regulator protein (CFTR) and its natural splice variant, TNR-CFTR. To accomplish this, Madin-Darby canine kidney (MDCK) stable cell lines expressing TNR-CFTR or CFTR were used. The cells were treated with hypertonic medium made with either NaCl or urea or sucrose (480 mOsm/kg or 560 mOsm/kg) to mimic the tonicity of the renal medulla environment. Western blot data showed that CFTR and TNR-CFTR total cell protein is increased by hypertonic medium, but using the surface biotinylation technique, only CFTR was found to be increased in cell plasma membrane. Confocal microscopy showed TNR-CFTR localization primarily at the endoplasmic reticulum and plasma membrane. In conclusion, CFTR and TNR-CFTR have different patterns of distribution in MDCK cells and they are modulated by a hypertonic environment, suggesting their physiological importance in renal medulla.
机译：这项研究检验了以下假设，即肾髓质的高渗环境调节了囊性纤维化跨膜电导调节蛋白（CFTR）及其天然剪接变体TNR-CFTR的表达。为此，使用了表达TNR-CFTR或CFTR的Madin-Darby犬肾（MDCK）稳定细胞系。用由NaCl或尿素或蔗糖制成的高渗培养基（480 mOsm / kg或560 mOsm / kg）处理细胞，以模拟肾髓质环境的张力。蛋白质印迹数据表明，高渗介质可增加CFTR和TNR-CFTR的总细胞蛋白，但使用表面生物素化技术，仅在细胞质膜中发现CFTR会增加。共聚焦显微镜检查显示，TNR-CFTR主要位于内质网和质膜。总之，CFTR和TNR-CFTR在MDCK细胞中具有不同的分布模式，并且受到高渗环境的调节，表明它们在肾髓质中的生理重要性。

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