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首页> 外文期刊>Cellular Physiology and Biochemistry >FTY720-Induced Suicidal Erythrocyte Death
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FTY720-Induced Suicidal Erythrocyte Death

机译：FTY720诱导的自杀性红细胞死亡

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FTY720 is a potent anti-inflammatory drug known to trigger suicidal death or apoptosis of a variety of nucleated cells. Erythrocytes may similarly undergo suicidal erythrocyte death or eryptosis. Hallmarks of eryptosis include cell membrane scrambling and cell shrinkage, which are triggered by increase in cytosolic Casup2+/sup concentration and ceramide. The present study explored whether FTY720 stimulates eryptosis. Cell membrane scrambling was determined from annexin V-binding, cell shrinkage from forward scatter in FACS analysis, cytosolic Casup2+/sup concentration from Fluo3 fluorescence, ceramide formation from fluorescence-labeled antibody binding and hemolysis from the hemoglobin concentration in the supernatant. Within 48 hours exposure to FTY720 (10 µM) significantly increased annexin V-binding, decreased forward scatter and increased cytosolic Casup2+/sup concentration but did not significantly modify ceramide formation. The effects of FTY720 were significantly blunted in the nominal absence of extracelluar Casup2+/sup. In conclusion, at toxic concentrations, FTY720 stimulates suicidal cell death, an effect at least partially due to stimulation of Casup2+/sup entry.
机译：FTY720是一种有效的抗炎药，已知会引起多种有核细胞自杀死亡或凋亡。红细胞可能类似地经历自杀性红细胞死亡或隐匿。密码学的特征包括细胞膜混乱和细胞收缩，这是由胞质Ca 2 + 浓度和神经酰胺增加引起的。本研究探讨了FTY720是否刺激加密。根据膜联蛋白V结合，FACS分析中向前散射的细胞收缩，Fluo3荧光的胞浆Ca 2 + 浓度，荧光标记的抗体结合产生的神经酰胺形成以及血红蛋白浓度的溶血来确定细胞膜的扰动在上清液中。在暴露于FTY720（10 µM）的48小时内，膜联蛋白V结合显着增加，向前散射降低，胞质Ca 2 + 浓度增加，但并未显着改变神经酰胺的形成。在名义上不存在细胞外Ca 2 + 的情况下，FTY720的作用明显减弱。总之，在有毒浓度下，FTY720刺激自杀性细胞死亡，这种作用至少部分是由于刺激Ca 2 + 进入。

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