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首页> 外文期刊>Ceylon Medical Journal >Validating the Loop Mediated Isothermal Amplification (LAMP) technique to detect tuberculosis in a Sri Lankan laboratory setting
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Validating the Loop Mediated Isothermal Amplification (LAMP) technique to detect tuberculosis in a Sri Lankan laboratory setting

机译：在斯里兰卡的实验室环境中验证回路介导的等温扩增（LAMP）技术以检测结核病

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摘要

A critical obstacle to control tuberculosis (TB) causedby Mycobacterium tuberculosis (MTB) in the region isthe lack of early and accurate diagnosis. The currentlyavailable methods for TB diagnosis, such as microscopy,culture, and PCR are either time consuming or less suitablefor resource-limited rural settings. Previous studies haveattempted to validate the Loop Mediated IsothermalAmplification (LAMP) assay as an alternative assay forTB diagnosis, as it is a simple and less time consumingtechnique that is more suitable for developing countries[1-4]. However, success has been limited due to a recurrentnon-specific amplification of primers, leading to falsepositive results [5]. This paper describes the optimizationof the LAMP assay, to avoid this false positive detectionof MTB, and its efficacy as compared to the standardculture technique.
机译：控制该地区结核分枝杆菌（MTB）引起的结核病（TB）的关键障碍是缺乏早期和准确的诊断。目前可用的结核病诊断方法，如显微镜检查，培养和PCR，既费时又不适合资源有限的农村地区。先前的研究试图验证环介导的等温扩增（LAMP）试验作为结核病诊断的替代方法，因为它是一种简单且耗时较少的技术，更适合于发展中国家[1-4]。然而，由于引物的反复非特异性扩增，成功受到了限制，导致假阳性结果[5]。与标准培养技术相比，本文描述了LAMP检测的优化方法，以避免MTB的假阳性检测，以及其功效。

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