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首页> 外文期刊>CHROMATOGRAPHY >Analysis of Fusarium Toxins in Processed Grain Products Using High-Performance Liquid Chromatography/Tandem Mass Spectrometry
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Analysis of Fusarium Toxins in Processed Grain Products Using High-Performance Liquid Chromatography/Tandem Mass Spectrometry

机译：高效液相色谱/串联质谱法分析谷物制品中的毒素

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摘要

An analytical method for the simultaneous detection of five types of Fusarium toxins (nivalenol (NIV), deoxynivalenol (DON), HT-2 toxin (HT-2), T-2 toxin (T-2), and zearalenone (ZEN)) in processed grains has been developed. Mycotoxins in the sample were extracted using acetonitrile-water (85:15 v/v), purified using MultiSep 226 AflaZon+ multi-functional cartridge columns, and quantified using high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS). With wheat flour, the proposed method showed good linearity (r > 0.999), and the accuracy was 85 - 97%. The RSD values of the intra-day precision were 1.9 - 13.3% and those of the inter-day precision were 10.2 - 19.6%. In a study of commercial foodstuffs, the limit of quantification was 15 μg/kg for NIV and DON, and 5 μg/kg for HT-2, T-2, and ZEN. Using the validated method, we conducted a contamination study of 55 processed food samples, including a variety of wheat and corn products. DON was detected in 36 samples, with a maximum concentration of 238 μg/kg in corn grits. In addition, HT-2 was detected in six samples, and T-2 was detected in two samples, in products that contained wheat as the raw material, with concentrations of less than 20 μg/kg.
机译：同时检测五种F毒素的方法：萘烯醇（NIV），脱氧萘烯醇（DON），HT-2毒素（HT-2），T-2毒素（T-2）和玉米赤霉烯酮（ ZEN））已开发出来。使用乙腈-水（85:15 v / v）提取样品中的霉菌毒素，使用MultiSep 226 AflaZon +多功能柱色谱柱纯化，并使用高效液相色谱/串联质谱（LC-MS / MS）进行定量。对于小麦面粉，所提出的方法显示出良好的线性（r＞ 0.999），并且准确度为85-97％。日内精度的RSD值为1.9-13.3％，日间精度的RSD值为10.2-19.6％。在一项商业食品研究中，NIV和DON的定量限为15μg/ kg，HT-2，T-2和ZEN的定量限为5μg/ kg。使用经过验证的方法，我们对55种加工食品样本（包括各种小麦和玉米产品）进行了污染研究。在36个样品中检测到DON，玉米corn中的最大浓度为238μg/ kg。此外，在以小麦为原料的产品中，在六个样品中检测到HT-2，在两个样品中检测到T-2，浓度低于20μg/ kg。

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