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Compositions, Methods, and Kits for One-Step Digestion of Nucleic Acid for Analysis by Liquid Chromatography Tandem Mass Spectrometry
Compositions, Methods, and Kits for One-Step Digestion of Nucleic Acid for Analysis by Liquid Chromatography Tandem Mass Spectrometry
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机译:用于液相色谱串联质谱分析的一步一步消化核酸的组合物,方法和试剂盒
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摘要
A kit for preparing a nucleic acid sample for analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) is provided, the kit comprising: a lyophilized enzyme composition comprising: micrococcal nuclease; nuclease P1; and bacterial alkaline phosphatase (BAP); and a digestion buffer. Also provided are enzyme compositions and methods of use for rapid, efficient preparation of a nucleic acid sample for analysis by LC-MS/MS, without the need for denaturation of the sample.
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