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Compositions, Methods, and Kits for One-Step Digestion of Nucleic Acid for Analysis by Liquid Chromatography Tandem Mass Spectrometry

机译:用于液相色谱串联质谱分析的一步一步消化核酸的组合物,方法和试剂盒

摘要

A kit for preparing a nucleic acid sample for analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) is provided, the kit comprising: a lyophilized enzyme composition comprising: micrococcal nuclease; nuclease P1; and bacterial alkaline phosphatase (BAP); and a digestion buffer. Also provided are enzyme compositions and methods of use for rapid, efficient preparation of a nucleic acid sample for analysis by LC-MS/MS, without the need for denaturation of the sample.
机译:提供了一种用于制备通过液相色谱串联质谱法(LC-MS / MS)分析的核酸样品的试剂盒,该试剂盒包括:冻干的酶组合物,其包含:微球菌核酸酶;和核酸酶P1;和细菌碱性磷酸酶(BAP);和消化缓冲液。还提供了酶组合物和用于快速,有效地制备核酸样品以通过LC-MS / MS进行分析而无需变性的酶组合物和使用方法。

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