• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Molecules >Comparative Analysis of Carbohydrates Nucleosides and Amino Acids in Different Parts of Trichosanthes kirilowii Maxim. by (Ultra) High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and Evaporative Light Scattering Detector Methods
【2h】

Comparative Analysis of Carbohydrates Nucleosides and Amino Acids in Different Parts of Trichosanthes kirilowii Maxim. by (Ultra) High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and Evaporative Light Scattering Detector Methods

机译：毛滴虫不同部位碳水化合物核苷和氨基酸的比较分析。 （超）高效液相色谱-串联质谱和蒸发光散射检测器分析

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Trichosanthes kirilowii Maxim. is one of the original plants for traditional Chinese medicines Trichosanthis Fructus, Trichosanthis Semen, Trichosanthis Pericarpium and Trichosanthis Radix. Amino acids, nucleosides and carbohydrates are usually considered to have nutritional value and health-care efficacy. In this study, methods involving high-performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD), UV-visible spectrophotometry and ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) were established for quantifying carbohydrates (fructose, glucose, stachyose, raffinose and polysaccharide), fourteen nucleosides and twenty one amino acids. Moreover, sixty-three samples from nine different parts, including pericarp, seed, fruit pulp, stem, leaf, main root, main root bark, lateral root and lateral root bark of T. kirilowii from different cultivated varieties were examined. The established methods were validated with good linearity, precision, repeatability, stability, and recovery. The results showed that the average content of total amino acids in roots (15.39 mg/g) and root barks (16.38 mg/g) were relatively higher than for others. Contents of nucleosides in all parts of T. kirilowii were below 1.5 mg/g. For carbohydrates, fruit pulp has a higher content than others for glucose (22.91%), fructose (20.63%) and polysaccharides (27.29%). By using partial least-squared discriminate analysis (PLS-DA), Variable importance in the projection (VIP) plots and analysis of variance (ANOVA) analysis, the characteristic components of the different organs (fruit, stems and leaves, roots) were found. This analysis suggested there were potential medicinal and nutritive health care values in various parts of the T. kirilowii, which provided valuable information for the development and utilization of T. kirilowii.
机译：Trichosanthes kirilowi​​i Maxim。是中药Tri藜，Tri实，Se皮，and皮的原始植物之一。通常认为氨基酸，核苷和碳水化合物具有营养价值和保健功效。在这项研究中，建立了涉及高效液相色谱结合蒸发光散射检测器（HPLC-ELSD），紫外可见分光光度法和超高效液相色谱结合串联质谱（UHPLC-MS / MS）的方法。定量碳水化合物（果糖，葡萄糖，水苏糖，棉子糖和多糖），十四个核苷和二十一个氨基酸。此外，还对来自不同栽培品种的麒麟菜的果皮，种子，果肉，茎，叶，主根，主根皮，侧根和侧根皮的九个不同部位的六十三份样品进行了检测。所建立的方法具有良好的线性，精密度，可重复性，稳定性和回收率。结果表明，根中总氨基酸的平均含量（15.39 mg / g）和根皮中的平均氨基酸含量（16.38 mg / g）相对较高。桐木所有部分的核苷含量均低于1.5 mg / g。对于碳水化合物，果肉中葡萄糖（22.91％），果糖（20.63％）和多糖（27.29％）的含量更高。通过使用偏最小二乘判别分析（PLS-DA），投影图（VIP）图中的变量重要性和方差分析（ANOVA）分析，发现了不同器官（水果，茎，叶，根）的特征成分。这项分析表明，在T. kirilowi​​i的各个部位都有潜在的医学和营养保健价值，这为T. kirilowi​​i的开发和利用提供了有价值的信息。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号