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首页> 外文期刊>CHROMATOGRAPHY >The Influence of Chyle and Bilirubin on Human Plasma Amino Acid Analysis by High-Performance Liquid Chromatography Ionization Mass Spectrometry
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The Influence of Chyle and Bilirubin on Human Plasma Amino Acid Analysis by High-Performance Liquid Chromatography Ionization Mass Spectrometry

机译：高效液相色谱电离质谱法研究胆红素和胆红素对人体血浆氨基酸的影响

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摘要

Plasma amino acid concentrations have been analyzed by high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI/MS) after precolumn derivatization. However, it is still unknown whether chyle and bilirubin, which are known to interfere with quantifications of some clinical biomarkers, have any influence on plasma amino acid measurement. In this study, we investigated the effects of those interference substances on amino acid concentrations determined by HPLC-ESI/MS. A total of 20 Japanese subjects (volunteers working for Ajinomoto Co., Inc.) were enrolled in this study. Twenty-one amino acid concentrations in plasma were measured with HPLCESI/MS after addition of each interference substances (a soy bean oil-egg yolk lecithin for the evaluation of chyle and total bilirubin). No significant changes of amino acid concentrations were observed by the addition of soybean oil (less than 0.25%) and bilirubin (final concentration: 0.5-2.0 mg/dL). Although the addition of 0.5% of soybean oil increased proline and phenylalanine levels significantly, the changes were less than ± 5% compared with blank samples. These results suggest that there would be little effects of chyle and bilirubin on the measurement of amino acid concentrations determined by the present HPLC-ESI/MS system.
机译：柱前衍生化后，通过高效液相色谱-电喷雾电离质谱（HPLC-ESI / MS）分析了血浆氨基酸浓度。但是，尚不清楚已知会干扰某些临床生物标志物定量的乳糜和胆红素对血浆氨基酸的测定是否有影响。在这项研究中，我们研究了这些干扰物质对通过HPLC-ESI / MS测定的氨基酸浓度的影响。本研究共招募了20名日本受试者（在Ajinomoto Co.，Inc.工作的志愿者）。加入每种干扰物质（用于评估乳糜和总胆红素的大豆油-蛋黄卵磷脂）后，用HPLCESI / MS测量血浆中的21个氨基酸浓度。通过添加大豆油（小于0.25％）和胆红素（终浓度：0.5-2.0 mg / dL），未观察到氨基酸浓度的显着变化。尽管添加0.5％的大豆油可显着提高脯氨酸和苯丙氨酸的水平，但与空白样品相比，变化小于±5％。这些结果表明，乳糜和胆红素对通过本HPLC-ESI / MS系统测定的氨基酸浓度的测量几乎没有影响。

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