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A Simple and Rapid Immunoassay Based on Microchip Electrophoresis Using a Reagent-Release Cartridge

机译：基于试剂释放盒的Microchip电泳的快速简便的免疫测定

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摘要

In this article, we report a simple and rapid immunoassay based on microscale electrophoresis using a microchip equipped with a reagent-release cartridge at the sample reservoir. The cartridge consists of an outer tube, a paper filter retaining dried reagents and an antibody, and inner tubes to fix the paper filter. When a sample solution containing an antigen is introduced into the sample reservoir through the reagent-release cartridge, the reagents and the antibody retained on the filter dissolve immediately into the sample solution. The sample solution and reagents containing the antibody are then mixed, promoting rapid immunoreaction between the antigen and the antibody. After sample introduction into the reservoir, the antigen-antibody complex is rapidly separated from the free antibody by microchip zone electrophoresis. The immunoassays for bovine serum albumin and human IgG were easily performed merely by pouring the sample solution into the cartridge in the newly developed device followed by microchip electrophoresis, resulting in the rapid and clear separation of the immunocomplexes from the free antibodies. These results showed that the developed device was applicable to a rapid and simple immunoassay.
机译：在本文中，我们报告了一种基于微尺度电泳的简单，快速的免疫测定方法，该方法使用了在样品池中配备有试剂释放盒的微芯片。墨盒由外管，保留干燥试剂和抗体的滤纸和固定滤纸的内管组成。当通过试剂释放盒将包含抗原的样品溶液引入样品容器时，保留在过滤器上的试剂和抗体立即溶解在样品溶液中。然后将样品溶液和含有抗体的试剂混合，以促进抗原和抗体之间的快速免疫反应。将样品引入储器后，通过微芯片区电泳将抗原-抗体复合物与游离抗体快速分离。只需将样品溶液倒入新开发的设备中的药筒中，然后进行微芯片电泳，即可轻松进行牛血清白蛋白和人IgG的免疫分析，从而可将免疫复合物与游离抗体快速而清晰地分离。这些结果表明，所开发的装置适用于快速简单的免疫测定。

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