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Analysis of Psychoactive Drugs by Temperature-Responsive Chromatography

机译：温度响应色谱法分析精神活性药物

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Temperature-responsive chromatography, in which the characteristics of the stationary phase can be controlled by varying the column temperature with only an aqueous eluent, was applied to the analysis of psychoactive drugs. Temperature-responsive poly(N -isopropylacrylamide) (PNIPAAm)-based copolymers were synthesized with n -butyl methacrylate (BMA) and N -acryloyl L-phenylalanine methyl ester (Phe-OMe) as a comonomer. These polymers were grafted onto aminopropyl silica and used as the stationary phase. Seven psychoactive drugs could be separated simultaneously by the column, including BMA. Among the seven drugs, for triazolam, which has a triazole ring in its structure, the order of elution changed on changing the column temperature. The phenomenon could be explained by NH–π interactions between the NH groups of PNIPAAm and π electrons of the triazole ring. The conformational changes of PNIPAAm altered the degree of exposure of the NH groups and would affect the elution order of the analytes. To enhance the molecular recognition ability for the triazole ring, the column containing Phe-OMe was used. Noticeable changes in the retention factors occurred due to the additional π–π interactions between the phenyl moieties of Phe-OMe and the triazole ring. The effects of the π–π interactions were also altered by the changes in column temperature. This chromatographic system, which needs no toxic organic solvent, offers an effective way to determine the causes of addiction in medical institutions.
机译：温度响应色谱法可用于分析精神活性药物，在该方法中，仅用水性洗脱液即可通过改变色谱柱温度来控制固定相的特性。以甲基丙烯酸正丁酯（BMA）和丙烯酸N-丙烯酰基L-苯丙氨酸甲酯（Phe-OMe）为原料，合成了基于温度响应性的聚（N-异丙基丙烯酰胺）（PNIPAAm）基共聚物。共聚单体。这些聚合物被接枝到氨基丙基二氧化硅上并用作固定相。该色谱柱可以同时分离出七种精神药物，包括BMA。在这七种药物中，对于结构上具有三唑环的三唑仑，洗脱顺序随柱温的改变而改变。该现象可以通过PNIPAAm的NH基团与三唑环的π电子之间的NH-π相互作用来解释。 PNIPAAm的构象变化会改变NH基团的暴露程度，并会影响分析物的洗脱顺序。为了增强对三唑环的分子识别能力，使用了包含Phe-OMe的色谱柱。由于Phe-OMe的苯基部分与三唑环之间存在额外的π-π相互作用，因此保留因子发生了显着变化。柱温的变化也改变了π-π相互作用的影响。该色谱系统不需要有毒的有机溶剂，提供了一种确定医疗机构成瘾原因的有效方法。

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