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首页> 外文期刊>Clinical and vaccine immunology: CVI >Functional and Specific Antibody Responses in Adult Volunteers in New Zealand Who Were Given One of Two Different Meningococcal Serogroup B Outer Membrane Vesicle Vaccines
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Functional and Specific Antibody Responses in Adult Volunteers in New Zealand Who Were Given One of Two Different Meningococcal Serogroup B Outer Membrane Vesicle Vaccines

机译：新西兰成人志愿者的功能和特异性抗体反应，被给予两种不同的脑膜炎球菌血清群B外膜囊泡疫苗之一

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This study presents detailed analyses of total and specific serum antibody levels among 26 and 24 adult volunteers before vaccination and after the third dose of the meningococcal serogroup B outer membrane vesicle (OMV) vaccines MeNZB and MenBvac, respectively, in a clinical trial in New Zealand (V. Thornton, D. Lennon, K. Rasanathan, J. O'Hallahan, P. Oster, J. Stewart, S. Tilman, I. Aaberge, B. Feiring, H. Nokleby, E. Rosenqvist, K. White, S. Reid, K. Mulholland, M. J. Wakefield, and D. Martin, Vaccine 24:1395-1400, 2006). With the homologous vaccine strains as targets, both vaccines induced significant increases in serum bactericidal and opsonophagocytic activities and in the levels of immunoglobulin G (IgG) to OMV antigens in an enzyme-linked immunosorbent assay (ELISA) and to live meningococci by flow cytometry. They also induced high levels of activity against the heterologous strains, particularly in terms of opsonophagocytic activity and IgG binding to live bacteria. The antibody levels with the homologous and heterologous strains in the four assays showed high and significant positive correlations. Specific IgG binding to 10 major OMV antigens in each vaccine was measured by scanning of immunoblots; ELISAs for two antigens, lipopolysaccharide and Neisseria surface protein A (NspA), were also performed. Both vaccines elicited significant increases in IgG binding to all homologous and heterologous OMV antigens except NspA. The total IgG band intensity on the blots correlated significantly with the IgG levels determined by the OMV ELISA and flow cytometry. In conclusion, the results of the various immunological assays showed that both OMV vaccines gave rise to high levels of specific and cross-reacting antibodies.
机译：这项研究在新西兰的一项临床试验中分别对26例和24例成年志愿者在疫苗接种前和第三剂脑膜炎球菌B群血清外膜囊泡（OMV）疫苗MeNZB和MenBvac进行了总体和特异性血清抗体水平的详细分析（V.Thornton，D.Lennon，K.Rasanathan，J.O'Hallahan，P.Oster，J.Stewart，S.Tilman，I.Aaberge，B.Feiring，H.Nokleby，E.Rosenqvist，K.White ，S。Reid，K。Mulholland，MJ Wakefield和D. Martin，疫苗24：1395-1400，2006年）。以同源疫苗株为靶标，两种疫苗均通过酶联免疫吸附测定（ELISA）诱导了血清杀菌和调理吞噬细胞活性以及OMV抗原的免疫球蛋白G（IgG）水平的显着提高，并通过流式细胞术检测了活的脑膜炎球菌。他们还诱导了针对异源菌株的高水平活性，特别是在调理吞噬细胞活性和与活细菌结合的IgG方面。在四种测定中，具有同源和异源菌株的抗体水平显示出高和显着的正相关。通过扫描免疫印迹来测量与每种疫苗中的10种主要OMV抗原结合的特异性IgG;还对两种抗原（脂多糖和奈瑟菌表面蛋白A（NspA））进行了ELISA。两种疫苗均引起与NspA以外的所有同源和异源OMV抗原的IgG结合的显着增加。印迹上的总IgG带强度与通过OMV ELISA和流式细胞仪确定的IgG水平显着相关。总之，各种免疫测定的结果表明，两种OMV疫苗均可产生高水平的特异性和交叉反应抗体。

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