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首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >Assessment of intact Phospholipids in Outer Membrane Vesicles of Neisseria meningitidis Serogroup B Bacteria with nanoscale LC-MS
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Assessment of intact Phospholipids in Outer Membrane Vesicles of Neisseria meningitidis Serogroup B Bacteria with nanoscale LC-MS

机译：用纳米级LC-MS评估Neisseria Meningitidis Serogroup B细菌外膜囊泡的完整磷脂

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摘要

Meningococcal Outer Membrane Vesicles (OMV) are important vaccine candidates against serogroup B meningococci, as these OMVs expose the most important membrane protein (Porin A) that act as primary targets for bactericidal antibodies. The membrane of the OMV is a bilayer composed of different phospholipids. Characterization of these phospholipid constituents is of strategic importance in the OMV production and as part of the validation process of the vaccine product. Due to their amphiphilic nature, chromatographic separation of phospholipids is difficult in terms of separation efficiency, resolution and sensitivity. We present a reversed-phase nanoscale LC-MS technique to determine different phospholipids, based on a system developed in our group and routinely used nowadays for over a decade in peptide and protein analysis.
机译：脑膜炎球菌外膜囊泡（OMV）是对血清群体B脑膜炎的重要疫苗候选，因为这些OMV暴露了充当杀菌抗体的主要靶标的最重要的膜蛋白（Porin A）。 OMV的膜是由不同磷脂组成的双层。这些磷脂成分的表征在OMV生产中具有战略性重要性，以及作为疫苗产品的验证过程的一部分。由于它们的两亲性质，在分离效率，分辨率和敏感性方面，磷脂的色谱分离难以。我们提出了一种逆转阶段纳米级LC-MS技术，以确定不同的磷脂，基于我们的组中开发的系统，并在目前在肽和蛋白质分析中经常使用过度使用。

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