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首页> 外文期刊>African Journal of Microbiology Research >Outer membrane vesicle of Neisseria meningitidis serogroup B as an adjuvant in immunization of rabbit against Neisseria meningitidis serogroup A
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Outer membrane vesicle of Neisseria meningitidis serogroup B as an adjuvant in immunization of rabbit against Neisseria meningitidis serogroup A

机译：Neisseria Meningitidis血清群B的外膜囊泡作为兔子免疫兔子免疫接种脑膜脑霉菌血清群A辅助剂

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Neisseria meningitidis?serogroup B outer membrane vesicle (OMV) was revealed to be an efficient carrier for polysaccharide antigens such as capsular antigens. Also, OMV of?N. meningitidis?was shown to be able to induce high levels of antibodies when applied as an adjuvant for T-independent antigens. In this study OMV was used as an adjuvant with group A meningococcal capsular polysaccharide (GAMP) and tested in New zealand white rabbits to evaluate bactericidal antibody response and opsonophagocytosis activity against serogroup A meningococci. Non-covalent combination of OMV and GAMP and three controls including GAMP, OMV and normal saline were injected intramuscularly into groups of four female New Zealand rabbits with boosters on days 14 and 28 after primary immunization. The serum samples were collected on days 0, 14, 28 and 42 and tested by complement mediated bactericidal assay and opsonophagocytosis activity against serogroup A meningococci according to the world health organization protocol. The results indicate that the combination of OMV with GAMP, in noncovalent form, would be able to induce a high level of bactericidal antibody and opsonophagocytosis activity response in comparison with GAMP alone after 42 days (P< 0.05).? The OMV of?N. meningitidis?showed to be a potent carrier protein in the induction of immune system but in this article the role of OMV is studied as an adjuvant to promote immune system in non-covalent form and without any conjugation process in order to induce immune response against three prevalent serogroups of?N. meningitidis.
机译：Neisseria Meningitidis？血清群B外膜囊泡（OMV）被揭示为具有多糖抗原的有效载体，例如囊状抗原。另外，omv的omv。脑蓝芽虫？当施加为邻无抗原的佐剂时，被证明能够诱导高水平的抗体。在本研究中，OMV被用作脑膜炎球菌荚膜多糖（GAMP）组的佐剂，并在新西兰白兔测试，以评估对血细胞的血清组的杀菌抗体反应和Opsonococococytiss活性。在初级免疫后，将肌肉注射成肌肉内，肌肉注射，将包括垃圾，omv和生理盐水在内的三个雌性新西兰兔的组合的非共价组合。根据世界卫生组织议定书，在第0,14,28和42天收集血清样品，并通过补体介导的杀菌测定和OPSONOCHAGONAGOSINS活性对脑细胞组织策略进行测试。结果表明，在非价形式中，OMV与垃圾中的组合能够诱导高水平的杀菌抗体和Opsonocococytiss活性反应，同时在42天后单独的垃圾（P <0.05）。 omv？n。脑膜炎？在免疫系统诱导中表明是一种有效的载体蛋白，但在本文中，研究了OMV的作用作为佐剂，以促进非共价形式的免疫系统，没有任何缀合过程以诱导三个免疫应答普遍的血清小组？n。脑膜炎。

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