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Delivery type not associated with global methylation at birth

机译：分娩类型与出生时的整体甲基化无关

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BackgroundBirth by cesarean delivery (CD) as opposed to vaginal delivery (VD) is associated with altered health outcomes later in life, including respiratory disorders, allergies and risk of developing type I diabetes. Epigenetic gene regulation is a proposed mechanism by which early life exposures affect later health outcomes. Previously, type of delivery has been found to be associated with differences in global methylation levels, but the sample sizes have been small. We measured global methylation in a large birth cohort to identify whether type of delivery is associated with epigenetic changes. MethodsDNA was isolated from cord blood collected from the University of Michigan Women’s & Children Hospital and bisulfite-converted. The Luminometric Methylation Assay (LUMA) and LINE-1 methylation assay were run on all samples in duplicate. ResultsGlobal methylation data at CCGG sites throughout the genome, as measured by LUMA, were available from 392 births (52% male; 65% CD), and quantitative methylation levels at LINE-1 repetitive elements were available for 407 births (52% male; 64% CD). LUMA and LINE-1 methylation measurements were negatively correlated in this population (Spearman’s r?=??0.13, p =0.01). LUMA measurements were significantly lower for total CD and planned CD, but not emergency CD when compared to VD (median VD?=?74.8, median total CD?=?74.4, p =?0.03; median planned CD?=?74.2, p =?0.02; median emergency CD?=?75.3, p =?0.39). However, this association did not persist when adjusting for maternal age, maternal smoking and infant gender. Furthermore, total CD deliveries, planned CD and emergency CD deliveries were not associated with LINE-1 measurements as compared to VD (median VD?=?82.2, median total CD?=?81.9, p =?0.19; median planned CD?=?81.9, p =?0.19; median emergency CD?=?82.1, p =?0.52). This lack of association held when adjusting for maternal age, maternal smoking and infant gender in a multivariable model. ConclusionsType of delivery was not associated with global methylation in our population, even after adjustment for maternal age, maternal smoking, and infant gender. While type of birth may be associated with later health outcomes, our data suggest that it does not do so through changes in global genomic methylation.
机译：背景剖宫产（CD）而非阴道分娩（VD）的出生与以后生活中健康状况的改变有关，包括呼吸系统疾病，过敏和罹患I型糖尿病的风险。表观遗传基因调控是一种提出的机制，通过该机制早期生命暴露会影响后期健康结果。以前，已经发现递送类型与总体甲基化水平的差异有关，但是样本量很小。我们在一个大的出生队列中测量了总体甲基化，以确定分娩的类型是否与表观遗传学变化有关。方法从密歇根大学妇女儿童医院收集的脐带血中分离DNA，并将其转化为亚硫酸氢盐。对所有样品一式两份进行发光甲基化测定（LUMA）和LINE-1甲基化测定。结果通过LUMA测量，可从392个出生（52％的男性; 65％的CD）获得整个基因组CCGG位点的总体甲基化数据，而407个出生（LINE％的男性; 52％的男性）可获得LINE-1重复元件的定量甲基化水平。 64％CD）。该人群的LUMA和LINE-1甲基化测量值呈负相关（Spearman的r？=？0.13，p = 0.01）。与VD相比，总CD和计划CD的LUMA测量值明显较低，但不是紧急CD（中值VD？=？74.8，中值总CD？=？74.4，p =？0.03;中值计划CD？=？74.2，p）。 = 0.02;紧急CD的中位数= 75.3，p = 0.39）。但是，在调整产妇年龄，产妇吸烟和婴儿性别时，这种关联并没有持续。此外，与VD相比，CD总交付量，计划CD和紧急CD交付量与LINE-1测量值无关（VD中位数=？82.2，CD总中位数=？81.9，p =？0.19;计划CD中位数=？ 81.9，p ＝ 0.19;紧急状态中位数CD ＝ 82.1，p ＝ 0.52。在多变量模型中调整产妇年龄，产妇吸烟和婴儿性别时，缺乏这种关联。结论即使调整了产妇年龄，产妇吸烟和婴儿性别，分娩类型也不与总体甲基化相关。虽然出生类型可能与以后的健康状况相关，但我们的数据表明，出生类型与整体基因组甲基化的改变无关。

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